School Of Basic And Applied Sciences

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    Genome-wide identification of NAC transcription factors in Avena sativa under salinity stress
    (Elsevier B.V., 2023-10-29T00:00:00) Bokolia, Muskan; Singh, Baljinder; Kumar, Avneesh; Goyal, Nandni; Singh, Kashmir; Chhabra, Ravindresh
    Background: NAC (NAM, ATAF1/2, and CUC2) is one of the most prominent family of plant-specific transcription factors that play diverse roles in plant growth and development as well as in abiotic stress responses in plants. The members of this family are recognized by presence of typical conserved NAC domain at the N-terminal and diverse C-terminal region. Results: In this study, we have identified 101 Avena sativa NAC (AsNAC) genes from the available Avena genome database. Genes were analyzed for their physicochemical properties, conserved motifs, gene structure, chromosomal localization, phylogenetic relationship, and cis-acting elements. The phylogenetic analysis illustrated that there were 15 subgroups in both Avena sativa and Arabidopsis thaliana. Mainly four types of cis-acting regulatory elements were present in the promoter regions of NAC genes, including hormone-responsive, light-responsive, stress-responsive and growth and developmental responsive elements. The chromosomal mapping analysis concluded that 101 NAC genes of Avena sativa were unevenly distributed on 21 chromosomes. Expression analysis identified 27 Avena NAC genes that respond to salt stress based on transcriptomic data analysis available on the NCBI SRA database. Significance: The genome-wide identification and molecular analysis of NAC TFs involved in environmental stress responses have the ability to overcome the limitations that came across in producing the transgenic crops with superior quality and improved production under abiotic stressed conditions. Future prospectives: These NAC genes may be considered as potential candidates for further explorations of functional analysis and could be used to develop stress tolerant lines in Avena sativa. � 2023 The Author(s)
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    Preconditioning of chickpea seedlings for terminal heat stress: understanding associated mechanism and HSP's expression
    (Central University of Punjab, 2014) Yadav, Renu; Kumar, Sanjeev
    Chickpea (Cicer arietinium L.) is the second most important pulse crop grown worldwide. Changes in the cropping system, competition from other cash crops (wheat) and global warming are pushing chickpea to relatively warmer growing environment. In northern part of country chickpea come across with terminal high temperature stress during reproductive stage which lead to reduced grain yield. Therefore to prevent the plant from incoming heat stress, 11 day chickpea seedling were preconditioned with mild drought stress, then put on recovery for six days and then recovered seedlings were exposed to lethal stress (where temperature was increased step wise from 30?C to 36?C). This study revealed that % EL, Lipid peroxidation increased with the increase in temperature while percent TTC reduction and total protein content decreased with the increase in temperature. Antioxidative enzymes provide the major defence against the ROS generated during the abiotic stress, and it was found that activity of SOD, CAT and APX enzyme increased proportionately with the rising temperature. HSP's act as molecular chaperons and are over expressed at both mRNA and protein level in preconditioned seedlings exposed to high temperature stress as compared to non- preconditioned ones. To conclude the whole study, results obtained clearly reveal that preconditioning with drought stress has the ability to improve tolerance above ambient temperature (27?C 7?C), thereafter preconditioning did not have any influence in terms of the improvement in membrane damage and level of antioxidants. Higher expression of sHSP's is corroborated with the low expression of antioxidants.