School Of Basic And Applied Sciences

Permanent URI for this communityhttps://kr.cup.edu.in/handle/32116/17

Browse

Subject: search.filters.subject.Immobilization

Search Results

Now showing 1 - 3 of 3
  • No Thumbnail Available
    Item
    Bioactive peptides for boosting stem cell culture platform: Methods and applications
    (Elsevier Masson s.r.l., 2023-02-09T00:00:00) Abdal Dayem, Ahmed; Lee, Soo Bin; Lim, Kyung Min; Kim, Aram; Shin, Hyun Jin; Vellingiri, Balachandar; Kim, Young Bong; Cho, Ssang-Goo
    Peptides, short protein fragments, can emulate the functions of their full-length native counterparts. Peptides are considered potent recombinant protein alternatives due to their specificity, high stability, low production cost, and ability to be easily tailored and immobilized. Stem cell proliferation and differentiation processes are orchestrated by an intricate interaction between numerous growth factors and proteins and their target receptors and ligands. Various growth factors, functional proteins, and cellular matrix-derived peptides efficiently enhance stem cell adhesion, proliferation, and directed differentiation. For that, peptides can be immobilized on a culture plate or conjugated to scaffolds, such as hydrogels or synthetic matrices. In this review, we assess the applications of a variety of peptides in stem cell adhesion, culture, organoid assembly, proliferation, and differentiation, describing the shortcomings of recombinant proteins and their full-length counterparts. Furthermore, we discuss the challenges of peptide applications in stem cell culture and materials design, as well as provide a brief outlook on future directions to advance peptide applications in boosting stem cell quality and scalability for clinical applications in tissue regeneration. � 2023 The Authors
  • No Thumbnail Available
    Item
    An Overview of Immunosensors and Their Application
    (Springer Nature, 2023-03-01T00:00:00) Gupta, Anil Kumar; Animesh, Sambhavi; Singh, Amit
    A key challenge in clinical healthcare is meeting the need to detect a disease at an early stage. Early and accurate diagnosis not only cuts the treatment cost but can also reduce disease burden, mortality rate, and social inequalities. Therefore, researchers are always searching for a method that allows rapid, simple, sensitive, selective, and cost-effective detection of the target biomarker (peptides, proteins, or nucleic acid). Immunosensors are one such point-of-care diagnostic device that can play an important role in almost all clinical healthcare fields. They are a promising alternative to the traditional immunoassays and state-of-the-art affinity sensors to diagnose clinically important analytes/antigens due to their high affinity, versatility, compact size, fast response time, minimum sample processing, and the measurements� reproducibility. For many decades now, significant advancement has been made in the immunosensor field in which the use of nanomaterials for increased sensitivity, multiplexing, or microfluidic-based devices may have the potential for promising use in clinical analysis. This chapter will provide an overview of the currently available immunosensor technology, its types that are currently being developed, and the limitations and future directions of immunosensor technology for the clinical laboratory. � The Editor (s) (if applicable) and the Author (s), under exclusive license to Springer Nature Singapore Pte Ltd.2023.
  • No Thumbnail Available
    Item
    An Overview of Immunosensors and Their Application
    (Springer Nature, 2023-03-01T00:00:00) Gupta, Anil Kumar; Animesh, Sambhavi; Singh, Amit
    A key challenge in clinical healthcare is meeting the need to detect a disease at an early stage. Early and accurate diagnosis not only cuts the treatment cost but can also reduce disease burden, mortality rate, and social inequalities. Therefore, researchers are always searching for a method that allows rapid, simple, sensitive, selective, and cost-effective detection of the target biomarker (peptides, proteins, or nucleic acid). Immunosensors are one such point-of-care diagnostic device that can play an important role in almost all clinical healthcare fields. They are a promising alternative to the traditional immunoassays and state-of-the-art affinity sensors to diagnose clinically important analytes/antigens due to their high affinity, versatility, compact size, fast response time, minimum sample processing, and the measurements� reproducibility. For many decades now, significant advancement has been made in the immunosensor field in which the use of nanomaterials for increased sensitivity, multiplexing, or microfluidic-based devices may have the potential for promising use in clinical analysis. This chapter will provide an overview of the currently available immunosensor technology, its types that are currently being developed, and the limitations and future directions of immunosensor technology for the clinical laboratory. � The Editor (s) (if applicable) and the Author (s), under exclusive license to Springer Nature Singapore Pte Ltd.2023.