School Of Basic And Applied Sciences

Permanent URI for this communityhttps://kr.cup.edu.in/handle/32116/17

Browse

Subject: search.filters.subject.Microvesicles

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Platelet-derived microvesicles activate human platelets via intracellular calcium mediated reactive oxygen species release
    (Academic Press Inc., 2022-08-28T00:00:00) Yadav, Pooja; Beura, Samir Kumar; Panigrahi, Abhishek Ramachandra; Bhardwaj, Taniya; Giri, Rajanish; Singh, Sunil Kumar
    Platelet-derived microvesicles (PMVs) are the most abundant microvesicles in circulation, originating from blood platelets via membrane blebbing. PMVs act as biological cargo carrying key molecules from platelets, including immunomodulatory molecules, growth factors, clotting molecules, and miRNAs that can regulate recipient cellular functions. Formation and release of PMVs play an essential role in the pathophysiology of vascular diseases such as hemostasis, inflammation, and thrombosis. Platelet activation is considered the critical event in thrombosis, and a growing number of evidence suggests that oxidative stress-mediated signaling plays a significant role in platelet activation. Ca2+ is a notable player in the generation of ROS in platelets. Reports have established that microvesicles exhibit dual nature in redox mechanisms as they possess both pro-oxidant and antioxidant machinery. However, the impact of PMVs and their ROS machinery on platelets is still a limited explored area. Here, we have demonstrated that PMVs mediate platelet activation via intracellular ROS generation. PMVs interacted with platelets and induced calcium-mediated intracellular ROS production via NADPH oxidase (NOX), leading to platelet activation. Our findings will open up new insights into the tangible relationship of PMVs with platelets and will further contribute to the therapeutic aspects of PMVs in vascular injury and tissue remodeling. � 2022
  • Thumbnail Image
    Item
    Development of lactadherin based electrochemical biosensor for the detection of platelet microvesicles
    (Central University of Punjab, 2018) Santra, Sneha; Singh, Sunil
    Platelet derived microvesicles (PMVs) are the most abundant membrane vesicles in the blood having a potent pro-inflammatory effect, promote coagulation and affect vascular function which are involved in the pathogenesis of cardiovascular disease including diabetes, thrombosis, and coronary artery diseases. Therefore, it is pertinent to detect PMVs level in blood of an individual which have prognostic potential for cardiovascular diseases. As per literature available, lactadherin is a small (53-66 kDa) multifunctional glycoprotein which plays an important role in the clearance of microvesicles. In the present study, lactadherin based electrochemical biosensor for the detection of PMVs was explored. Polythionine film (as a good electron mediator) was electrochemically deposited on ITO-coated glass through electrochemical process involving cyclic voltammetry (CV) and chronoamperometry. Electrochemically deposited electrode provides ideal adsorbing platform for immobilization of RGDS peptide sequences (Lactadherine binding motif) having binding affinity against active conformation of integrin on PMVs surface. CV and Diffusion Pulse Voltammetry (DPV) measurements showed gradual decrease of v current with the subsequent adsorption of microvesicles poor plasma (MPP), microvesicles rich plasma (MRP) and activated platelets. Decrease of current clearly depicts the presence of microvesicles in blood plasma. Our developed fabricated electrode can have a promising potential for its efficient application in clinical testing of various pathological conditions.