School Of Basic And Applied Sciences

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Subject: search.filters.subject.antimicrobial activity

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    Synthesis and Evaluation of Antimicrobial Activity of N-Substituted Indole Derivatives and Molecular Docking Studies
    (Bentham Science Publishers, 2022-11-18T00:00:00) Dwivedi, Ashish Ranjan; Kumar, Vijay; Neha; Jangid, Kailash; Devi, Bharti; Kulharia, Mahesh; Kumar, Rakesh; Kumar, Vinod
    The increasing burden of microbial infection and emerging resistance against the available antimicrobial drugs drives the development of new agents. Two different series of indole-based compounds (VN-1 to VN-18) were synthesized and analyzed for antimicrobial activity by calculating the diameter of the inhibition zone using the broth dilution method and well diffusion method against Escherichia coli (E. coli) and environmental microbes. Most of the compounds displayed good to moderate activity against E. coli, and VN-4 and VN-9 displayed good inhibitory activity against the tested microbes. Molecular docking and binding energy calculation studies of all the synthesized compounds have been performed for targeting FabI, where most of the compounds showed significant interactions with the aromatic nicotin-amide moiety of NAD+. In molecular dynamics studies, VN-9 stays inside the binding cavity for sufficient time to induce antimicrobial activity. Thus, these indole-based derivatives may lead to the development of new antimicrobi-al agents that may act as FabI inhibitors. � 2022 Bentham Science Publishers.
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    Antibacterial and Anticancer property of bioactive secondary metabolites from Actinomycetes isolated from different regions of Himachal Pradesh
    (Central University of Punjab, 2018) Chambiyal, Gourav; Kushawaha Pramod K.
    The prime objective of the present work is to isolate, characterize and purify soil microbes and to know about their antibacterial and anticancer properties. Soil samples were obtained from different locations in Dharamsala, Himachal Pradesh, India. Serial dilutions of the samples were made and plated on starch casein agar. Screening, purification and further tests were performed on the isolates to find the potential colonies. Biochemical and morphological characterization was done as well. Following characterization, isolates were used to extract bioactive secondary metabolites which can be anti-bacterial and anti-cancer. Secondary screening for antibacterial properties of isolates was evaluated by Agar well diffusion method. Further, the isolates were subjected to the GC-MS and MTT cytotoxic assay. Totally 7 isolates of actinomycetes were isolated and out of them only 4 were tested for antagonistic activity against 4 pathogenic microorganisms. Isolates M1,and M3 of dilution 10-1 were active, while M2 and M4 showed less activity against the pathogenic microorganisms. All actinomycetes isolates showed antibacterial activity against S. enterica and P. putida while they showed less activity against S. aureus and E.coli. These isolates showed antibacterial and anti-cancer activities and may be used for the growth of new antibiotics for pharmaceutical or agricultural purposes.