School Of Basic And Applied Sciences

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    A Systematic Role of Metabolomics, Metabolic Pathways, and Chemical Metabolism in Lung Cancer
    (MDPI, 2023-02-08T00:00:00) Kannampuzha, Sandra; Mukherjee, Anirban Goutam; Wanjari, Uddesh Ramesh; Gopalakrishnan, Abilash Valsala; Murali, Reshma; Namachivayam, Arunraj; Renu, Kaviyarasi; Dey, Abhijit; Vellingiri, Balachandar; Madhyastha, Harishkumar; Ganesan, Raja
    Lung cancer (LC) is considered as one of the leading causes of cancer-associated mortalities. Cancer cells� reprogrammed metabolism results in changes in metabolite concentrations, which can be utilized to identify a distinct metabolic pattern or fingerprint for cancer detection or diagnosis. By detecting different metabolic variations in the expression levels of LC patients, this will help and enhance early diagnosis methods as well as new treatment strategies. The majority of patients are identified at advanced stages after undergoing a number of surgical procedures or diagnostic testing, including the invasive procedures. This could be overcome by understanding the mechanism and function of differently regulated metabolites. Significant variations in the metabolites present in the different samples can be analyzed and used as early biomarkers. They could also be used to analyze the specific progression and type as well as stages of cancer type making it easier for the treatment process. The main aim of this review article is to focus on rewired metabolic pathways and the associated metabolite alterations that can be used as diagnostic and therapeutic targets in lung cancer diagnosis as well as treatment strategies. � 2023 by the authors.
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    Bacillus subtilis CP4, isolated from native soil in combination with arbuscular mycorrhizal fungi promotes biofortification, yield and metabolite production in wheat under field conditions
    (John Wiley and Sons Inc, 2020-12-03T00:00:00) Yadav, R.; Ror, P.; Rathore, P.; Kumar, S.; Ramakrishna, W.
    Aims: The aim of this study was to identify the best combination of plant growth promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF) for biofortification and enhancing yield in wheat as well as improve soil health under field conditions. Another aim was to get insights into metabolite dynamics in plants treated with PGPB and AMF. Methods and Results: Different combinations of PGPB and AMF that gave good results in greenhouse study were used in a field study. The combined application of Bacillus subtilis CP4 (native PGPB) and AMF gave the best results with a significant increase in biomass, macronutrient and micronutrient content in wheat grains and improvement in yield-related parameters relative to the untreated control. PGPB and AMF treatment increased antioxidant enzymes and compounds and decreased the level of an oxidation marker. Metabolite profiling performed using Gas Chromatography�Mass Spectrometry (GC-MS) showed significant upregulation of specific organic acids, amino acids, sugars and sugar alcohols in plants treated with CP4 and AMF. The altered pathways due to CP4 and AMF inoculation mainly belong to carbohydrate and amino acid metabolism. A positive correlation was observed between some organic acids, sugars and amino acids with wheat growth and yield parameters. The activities of soil enzymes increased significantly with the best results shown by native PGPB and AMF combination. Conclusions: A native bacterial isolate Bacillus subtilis CP4 in combination with AMF showed exceptional ability for biofortification and yield enhancement under field conditions. The upregulation of a number of metabolites showed correlation plant growth promotion and nutrients. Significance and Impact of the Study: The combined application of native B. subtilis CP4 and AMF could offer a more sustainable approach for the development of a biofertilizer to enhance wheat nutrient content and production and soil health thereby advancing agriculture. � 2020 The Society for Applied Microbiology
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    Bacillus subtilis CP4, isolated from native soil in combination with arbuscular mycorrhizal fungi promotes biofortification, yield and metabolite production in wheat under field conditions
    (John Wiley and Sons Inc, 2020-12-03T00:00:00) Yadav, R.; Ror, P.; Rathore, P.; Kumar, S.; Ramakrishna, W.
    Aims: The aim of this study was to identify the best combination of plant growth promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF) for biofortification and enhancing yield in wheat as well as improve soil health under field conditions. Another aim was to get insights into metabolite dynamics in plants treated with PGPB and AMF. Methods and Results: Different combinations of PGPB and AMF that gave good results in greenhouse study were used in a field study. The combined application of Bacillus subtilis CP4 (native PGPB) and AMF gave the best results with a significant increase in biomass, macronutrient and micronutrient content in wheat grains and improvement in yield-related parameters relative to the untreated control. PGPB and AMF treatment increased antioxidant enzymes and compounds and decreased the level of an oxidation marker. Metabolite profiling performed using Gas Chromatography�Mass Spectrometry (GC-MS) showed significant upregulation of specific organic acids, amino acids, sugars and sugar alcohols in plants treated with CP4 and AMF. The altered pathways due to CP4 and AMF inoculation mainly belong to carbohydrate and amino acid metabolism. A positive correlation was observed between some organic acids, sugars and amino acids with wheat growth and yield parameters. The activities of soil enzymes increased significantly with the best results shown by native PGPB and AMF combination. Conclusions: A native bacterial isolate Bacillus subtilis CP4 in combination with AMF showed exceptional ability for biofortification and yield enhancement under field conditions. The upregulation of a number of metabolites showed correlation plant growth promotion and nutrients. Significance and Impact of the Study: The combined application of native B. subtilis CP4 and AMF could offer a more sustainable approach for the development of a biofertilizer to enhance wheat nutrient content and production and soil health thereby advancing agriculture. � 2020 The Society for Applied Microbiology
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    Comparative Analysis of Metabolites in Contrasting Chickpea Cultivars
    (Central University of Punjab, 2018) Ghosh, Alokesh; Kumar, Vinay
    Phenolics are the major class of plant secondary metabolites. Among half of the plant phenolics are the flavonoids. The production of these metabolites induces during stresses and thus helps the plant to cope with the environment. Anthocyanins, a class of flavonoids protects plant from harmful UV rays, attracts the pollinators and seed dispersal, helps in mimicry and also helps in the root nodulation process in legumes. Chickpea is a leguminous and major pulse crop plant, shown in the winter season faces lots of stress. Anthocyanins and other phenolics help the plant to tolerate such stresses. Desi cultivars have more phenolics and anthocyanin as compared to the kabuli one. Flowers have higher concentration of anthocyanin than in leaves. Untargeted metabolomics profiling of the dry powdered mature seed samples of contrasting chickpea cultivars detects primary and secondary metabolites such as fatty acids, phenolics, phelols, terpenes, esters,sugar,etc in the seeds of chickpea.