Drought priming triggers diverse metabolic adjustments and induces chilling tolerance in chickpea (Cicer arietinum L.)

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dc.contributor.authorSaini, Rashmi
dc.contributor.authorAdhikary, Arindam
dc.contributor.authorJuneja, Sumandeep
dc.contributor.authorKumar, Rashpal
dc.contributor.authorSingh, Inderjit
dc.contributor.authorNayyar, Harsh
dc.contributor.authorKumar, Sanjeev
dc.date.accessioned2024-01-21T10:30:01Z
dc.date.accessioned2024-08-13T11:03:15Z
dc.date.available2024-01-21T10:30:01Z
dc.date.available2024-08-13T11:03:15Z
dc.date.issued2022-11-30T00:00:00
dc.description.abstractChickpea (Cicer arietinum L.) suffers from chilling stress at the reproductive stage (<15 �C) which leads to significant yield loss. This study presents a comprehensive plant response to drought priming and its effect on chilling tolerance during the reproductive stage in two chickpea cultivars PBG1 and PBG5. Lipidome profiling (Fatty acid methyl esters analysis), metabolome profiling (GC-MS based untargeted analysis), fatty acid desaturases and antioxidative gene expression (qRT-PCR) were analyzed to monitor physiological and biochemical events after priming during flowering, podding and seed filling stages. Drought priming alleviated membrane damage and chlorophyll degradation by increasing membrane unsaturated fatty acids (18:3) along with up-regulation of various fatty acid desaturases (CaFADs) genes and antioxidative machinery during flowering and improved seed yield in PBG5. PCA, HCA, and KEGG pathway analysis of 87 identified metabolites showed that metabolites were regulated differently in both cultivars under non-primed and primed conditions. The plant response was more apparent at flowering and podding stages which coincided with chilling temperature (<15 �C). Drought priming stimulated many important genes, especially FADs, antioxidative proteins and accumulation of key metabolites (proline and TCA intermediates) required for defense especially in PBG5. This explains that plant's response to drought priming not only depends on developmental stage, and temperature regime (<15 �C) but also on the genotypic-specificity. � 2022 Elsevier Masson SASen_US
dc.identifier.doi10.1016/j.plaphy.2022.11.034
dc.identifier.issn9819428
dc.identifier.urihttp://10.2.3.109/handle/32116/3174
dc.identifier.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0981942822005356
dc.language.isoen_USen_US
dc.publisherElsevier Masson s.r.l.en_US
dc.subjectAntioxidative defenseen_US
dc.subjectChilling stressen_US
dc.subjectDrought primingen_US
dc.subjectFatty acid desaturasesen_US
dc.subjectFatty acidsen_US
dc.subjectMetabolomicsen_US
dc.titleDrought priming triggers diverse metabolic adjustments and induces chilling tolerance in chickpea (Cicer arietinum L.)en_US
dc.title.journalPlant Physiology and Biochemistryen_US
dc.typeArticleen_US
dc.type.accesstypeOpen Accessen_US

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