SSR marker based DNA fingerprinting and diversity assessment in superior tea germplasm cultivated in western Himalaya

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dc.contributor.authorBhardwaj, Pankaj
dc.contributor.authorSharma, Rajesh Kumar
dc.contributor.authorKumar, Rajendra
dc.contributor.authorSharma, Hemlata
dc.contributor.authorAhuja, Paramvir Singh
dc.date.accessioned2019-04-29T10:37:22Z
dc.date.accessioned2024-08-13T11:02:35Z
dc.date.available2019-04-29T10:37:22Z
dc.date.available2024-08-13T11:02:35Z
dc.date.issued2014
dc.description.abstractTwenty one microsatellites (genomic & genic) markers were used to evaluate genetic diversity and DNA fingerprinting of 15 popular tea accessions. Each accession had a unique marker profile, indicating that microsatellite markers were useful in differentiation studies among the tea collections. A total of 127 polymorphic alleles were scored with an allele frequency of 6.05 per primer. The polymorphism information content (PIC) ranged from 0.2 (CamsinM1) to 0.60 (TUGMS12), with an average of 0.359. SSR markers analysis detected a high level of heterozygosity (av Ho 0.775; He 0.847) in tea. The Jaccard's similarity coefficients ranged from 0.15 to 0.56 with an average similarity index (ASI) of 0.234. The first two coordinate explained 54.33% of the total variance. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram and the PCoA (Principle coordinate analysis) indicated that the populations formed two major groups with exclusive China and China hybrids (I) and Assam types (II). The collections from western Himalayan possessed a moderate to high level of genetic diversity which could provide valid guidelines for genetic improvement of teaen_US
dc.identifier.citationBhardwaj, Pankaj., Sharma, Rajesh Kumar and Kumar, Rajendra et. al. (2014)SSR marker based DNA fingerprinting and diversity assessment in superior tea germplasm cultivated in western Himalaya. Proceedings of Indian National Science Academy. Vol. 80 (1), PP. 157-162en_US
dc.identifier.doi10.16943/ptinsa/2014/v80i1/55093
dc.identifier.issn2454-9983
dc.identifier.urihttp://10.2.3.109/handle/32116/2337
dc.identifier.urlhttps://insa.nic.in/UI/Journalarticle.aspx?jid=NA==&&VID=MTE=&&IsNm=SXNzdWUgMQ==
dc.language.isoenen_US
dc.publisherIndian National Academic Scienceen_US
dc.subjectAFLP: Amplified Length Polymorphismen_US
dc.subjectHe: Expected Hetrozygosityen_US
dc.subjectHo: Observed Heterozygosityen_US
dc.subjectTUGMS: Tea Unigene Micro Satelliteen_US
dc.subjectPIC: Polymorphism Information Contenten_US
dc.titleSSR marker based DNA fingerprinting and diversity assessment in superior tea germplasm cultivated in western Himalayaen_US
dc.title.journalProceedings of Indian National Science Academyen_US
dc.typeArticleen_US
dc.type.accesstypeOpen Accessen_US

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