Quantification and optimization of clot retraction in washed human platelets by Sonoclot coagulation analysis

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dc.contributor.authorYadav, Pooja
dc.contributor.authorBeura, Samir K.
dc.contributor.authorPanigrahi, Abhishek R.
dc.contributor.authorSingh, Sunil K.
dc.date.accessioned2024-01-21T10:44:37Z
dc.date.accessioned2024-08-13T13:21:42Z
dc.date.available2024-01-21T10:44:37Z
dc.date.available2024-08-13T13:21:42Z
dc.date.issued2021-10-07T00:00:00
dc.description.abstractIntroduction: Clot retraction is a pivotal process for haemostasis, where platelets develop a contractile force in fibrin meshwork and lead to the increased rigidity of clot. The pathophysiological alteration in contractile forces generated by the platelet-fibrin meshwork can lead to haemostatic disorders. Regardless of its utter significance, clot retraction remains a limited understood process owing to lack of quantification methodology. Sonoclot analysis is a point-of-care technique used in clinical laboratories for whole blood analysis that provides�in vitro�qualitative as well as quantitative assessment of coagulation process from initial fibrin formation to clot retraction. Methods: Human washed platelets were isolated by differential centrifugation method and analysed via optical imaging, microscopy and Sonoclot analysis using 1-2�נ108/mL of washed platelets, 1�U/mL of thrombin, 1�mg/mL of fibrinogen and 1�mM of calcium chloride. Results: In this study, we demonstrate the novelty of this instrument in the quantitative evaluation of clot retraction in washed platelets and attempted to optimize the reference range of Sonoclot parameters including ACT - 87.3���20.997, CR - 16.23���3.538 and PF - 3.57���0.629, (n�=�10). Discussion: Sonoclot analysis provides a simple and quantitative method to better understand in vitro clot retraction and its modulation by retraction components including platelet count, fibrinogen and platelet�fibrin interaction compared with existing conventional methods. Sonoclot may prove to be a valuable tool in thrombus biology research to understand fundamental basis of blood clot retraction. � 2021 John Wiley & Sons Ltden_US
dc.identifier.doi10.1111/ijlh.13710
dc.identifier.issn17515521
dc.identifier.urihttp://10.2.3.109/handle/32116/3809
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/10.1111/ijlh.13710
dc.language.isoen_USen_US
dc.publisherJohn Wiley and Sons Incen_US
dc.subjectBlood Coagulationen_US
dc.subjectBlood Coagulation Testsen_US
dc.subjectBlood Plateletsen_US
dc.subjectCalciumen_US
dc.subjectClot Retractionen_US
dc.subjectFlow Cytometryen_US
dc.subjectHealthy Volunteersen_US
dc.subjectHemostasisen_US
dc.subjectHumansen_US
dc.subjectMicroscopy, Phase-Contrasten_US
dc.subjectPlatelet Counten_US
dc.subjectPlatelet Function Testsen_US
dc.subjectcalcium chlorideen_US
dc.subjectfibrinogenen_US
dc.subjectthrombinen_US
dc.subjectcalciumen_US
dc.subjectArticleen_US
dc.subjectblood clot retractionen_US
dc.subjectcell isolationen_US
dc.subjectcentrifugationen_US
dc.subjectcontrolled studyen_US
dc.subjectflow cytometryen_US
dc.subjectfluorescence imagingen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectin vitro studyen_US
dc.subjectphase contrast microscopyen_US
dc.subjectplatelet counten_US
dc.subjectprocess optimizationen_US
dc.subjectquantitative analysisen_US
dc.subjectblooden_US
dc.subjectblood clottingen_US
dc.subjectblood clotting parametersen_US
dc.subjectblood clotting testen_US
dc.subjectdevicesen_US
dc.subjecthemostasisen_US
dc.subjectnormal humanen_US
dc.subjectproceduresen_US
dc.subjectthrombocyteen_US
dc.titleQuantification and optimization of clot retraction in washed human platelets by Sonoclot coagulation analysisen_US
dc.title.journalInternational Journal of Laboratory Hematologyen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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