Single-molecule force-unfolding of titin I27 reveals a correlation between the size of the surrounding anions and its mechanical stability

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dc.contributor.authorMuddassir, Mohd
dc.contributor.authorManna, Bharat
dc.contributor.authorSingh, Priyanka
dc.contributor.authorSingh, Surjeet
dc.contributor.authorKumar,Rajesh
dc.contributor.authorGhosh, Amit
dc.contributor.authorSharma, Deepak
dc.date.accessioned2019-03-26T09:01:39Z
dc.date.accessioned2024-08-13T11:16:30Z
dc.date.available2019-03-26T09:01:39Z
dc.date.available2024-08-13T11:16:30Z
dc.date.issued2018
dc.description.abstractEach cellular protein is surrounded by a biochemical milieu that affects its stability and the associated function. The role of this surrounding milieu in the proteins’ mechanical stability remains largely unexplored. Herein, we report an as yet unknown correlation between the size of the surrounding anions and the mechanical stability of a protein. Using single-molecule force spectroscopy of the 27th domain (I27) of human cardiac muscle protein titin, we show that the average unfolding force of the protein decreases with increase in the ionic radii of the surrounding anions in the order Cl− > Br− > NO3− > I− > SO42− ≈ ClO4−, indicating an inverse correlation between anion size and the mechanical stability of I27. The destabilizing effect was attributed to the combined effect of increase in the unfolding rate constant and unfolding distance upon incubation with the anion. These findings reveal that anion size can significantly affect the mechanical resistance of proteins and thus could be a convenient and promising tool for regulating the mechanical stability of proteins.en_US
dc.identifier.citationMuddassir, Mohd ., Manna, Bharat., Singh , Priyanka and et. al. (2018) Single-molecule force-unfolding of titin I27 reveals a correlation between the size of the surrounding anions and its mechanical stability. Chemical Communications. Vol. 54(69), PP. 9635-9638 . 10.1039/C8CC05557Ben_US
dc.identifier.doi10.1039/C8CC05557B
dc.identifier.issnPrint + online -1359-7345
dc.identifier.issnOnline-1364-548X
dc.identifier.urihttp://10.2.3.109/handle/32116/2169
dc.identifier.urlhttps://pubs.rsc.org/en/content/articlelanding/2018/cc/c8cc05557b/unauth#!divAbstract
dc.language.isoenen_US
dc.publisherRoyal Societty of chemistryen_US
dc.titleSingle-molecule force-unfolding of titin I27 reveals a correlation between the size of the surrounding anions and its mechanical stabilityen_US
dc.title.journalChemical Communicationsen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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