Mitigation of Gliadin-Induced Inflammation and Cellular Damage by Curcumin in Human Intestinal Cell Lines

dc.contributor.authorGupta, Kunj Bihari
dc.contributor.authorMantha, Anil K.
dc.contributor.authorDhiman, Monisha
dc.date.accessioned2024-01-21T10:44:34Z
dc.date.accessioned2024-08-13T13:22:19Z
dc.date.available2024-01-21T10:44:34Z
dc.date.available2024-08-13T13:22:19Z
dc.date.issued2021-01-04T00:00:00
dc.description.abstractWheat is a major diet from many years; apart from its nutritious value, the wheat protein gliadin is responsible for many inflammatory diseases like celiac disease (CD), and non-celiac gluten sensitivity (NCGS). In this study, the gliadin-induced inflammation and associated cellular damage along with the protective role of curcumin was evaluated using human intestinal cell lines (HCT-116 and HT-29) as a model. Cells were cultured and exposed to 160 ?g/ml of gliadin, 100 ?M H2O2, and 10 ?M curcumin (3 h pretreatment) followed by the assessment of inflammation. Spectrophotometric methods, real-time-PCR, ELISA, Western blotting, and confocal microscopy techniques were used to assess inflammatory markers such as advanced oxidation protein products (AOPPs) level, activity of myeloperoxidase (MPO) and NADPH oxidase (NOX), cytokines, and cell damage markers. The results show that gliadin increases the AOPPs level and the activity of MPO and NOX expression. It enhances inflammation by increasing expression of pro-inflammatory cytokines, altered expression of anti-inflammatory, and regulatory cytokines. It exacerbates the cellular damage by increasing MMP-2 and 9 and decreasing integrin ? and ? expression. Gliadin promotes disease pathogenesis by inducing the inflammation and cellular damage which further alter the cellular homeostasis. The pretreatment of curcumin counteracts the adverse effect of gliadin and protect the cells via diminishing the inflammation and help the cell to regain the cellular morphology suggesting phytochemical-based remedial interventions against wheat allergies. � 2021, Springer Science+Business Media, LLC, part of Springer Nature.en_US
dc.identifier.doi10.1007/s10753-020-01383-x
dc.identifier.issn3603997
dc.identifier.urihttps://kr.cup.edu.in/handle/32116/3794
dc.identifier.urlhttps://link.springer.com/10.1007/s10753-020-01383-x
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.subjectceliac diseaseen_US
dc.subjectcellular damageen_US
dc.subjectcurcuminen_US
dc.subjectgliadin proteinsen_US
dc.subjectinflammationen_US
dc.subjectqRT-PCRen_US
dc.titleMitigation of Gliadin-Induced Inflammation and Cellular Damage by Curcumin in Human Intestinal Cell Linesen_US
dc.title.journalInflammationen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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