Evaluation of leaves of Goniothalamus wynaadensis Bedd. for inhibition of metabolic viability of cancer cells & antimicrobial efficacy

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dc.contributor.authorSharma A.
dc.contributor.authorSharma P.
dc.contributor.authorSingh S.
dc.contributor.authorKaregoudar T.B.
dc.contributor.authorHolla H.
dc.date.accessioned2020-01-31T11:33:52Z
dc.date.accessioned2024-08-14T07:40:36Z
dc.date.available2020-01-31T11:33:52Z
dc.date.available2024-08-14T07:40:36Z
dc.date.issued2019
dc.description.abstractIntroduction: Goniothalamus wynaadensis Bedd. is a less explored medicinal plant belonging to the family Annonaceae. This species has been traditionally used by tribes of Wayanad, Kerala, in India, and also Indonesian tribes for joint-related ailments and also as a deodorizer. The aim of the study was to analyze leaf extracts of the plant for their antimicrobial and anticancer properties along with phytochemical screening. Methods: MTT assay was used to assess the cytotoxic potential of plant extracts against MDA-MB-231 and A-549. Antimicrobial potential of plant extracts was analyzed via liquid broth turbidometry assay and well in agar plate methods. Identification tests were carried out for phytochemical screening. Results: Antimicrobial assay showed EC50 values for ethyl acetate extract 0.82 mg/mL against Escherichia coli, 0.82 mg/mL against Salmonella typhi, & 0.88 mg/mL against Staphylococcus aureus which are quite significant. Other solvent extracts also displayed antimicrobial activities at varying dosage. MTT assay was performed at the dose concentrations of 1 ?g/mL, 5 ?g/mL & 25 ?g/mL, 50 ?g/mL and 100 ?g/mL to confirm the cytotoxicity of the extracts. The ethyl acetate extract displayed EC50 values of 4.96 ?g/mL against A-549 lung cancer cell line and 2.50 ?g/mL against MDA-MB-231 breast cancer cell line. Other extracts were also found to be cytotoxic. Conclusion: Ethyl acetate & water extracts have demonstrated potent activity against Salmonella typhi, Escherichia coli, & Staphylococcus aureus bacteria as well as, showing cytotoxicity against tested cancer cell lines. These results can be explored to identify individual phyto-molecules from the extracts with anticancer & antimicrobial potential.en_US
dc.identifier.doi10.1016/j.eujim.2019.101000
dc.identifier.issn18763820
dc.identifier.urihttp://10.2.3.109/handle/32116/2495
dc.identifier.urlhttps://www.sciencedirect.com/science/article/abs/pii/S1876382019306936
dc.language.isoen_USen_US
dc.publisherElsevier GmbHen_US
dc.subjectAnticancer activityen_US
dc.subjectAntimicrobial activityen_US
dc.subjectGoniothalamus wynaadensis Bedden_US
dc.subjectPhytochemical screeningen_US
dc.subjectSurvival indexen_US
dc.subjectTurbidometric analysisen_US
dc.titleEvaluation of leaves of Goniothalamus wynaadensis Bedd. for inhibition of metabolic viability of cancer cells & antimicrobial efficacyen_US
dc.title.journalEuropean Journal of Integrative Medicineen_US
dc.typeArticleen_US
dc.type.accesstypeClose Accessen_US

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