Anthocyanins enriched purple tea exhibits antioxidant, immunostimulatory and anticancer activities
Files
Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Springer India
Abstract
Purple coloured tea shoot clones have gained interest due to high content of anthocyanins in addition to catechins. Transcript expression of genes encoding anthocyanidin reductase (ANR), dihydroflavonol-4-reductase (DFR), anthocyanidin synthase (ANS), flavonol synthase (FLS) and leucoantho cyanidin reductase (LAR) enzymes in three new purple shoot tea clones compared with normal tea clone showed higher expression of CsDFR, CsANR, CsANS and lower expression of CsFLS and CsLAR in purple shoot clones compared to normal clone. Expression pattern supported high content of anthocyanins in purple tea. Four anthocyanins (AN1?4) were isolated and characterized by UPLC-ESI-QToF-MS/MS from IHBT 269 clone which recorded highest total anthocyanins content. Cyanidin-3-O-?-d-(6-(E)-coumaroyl) glucopyranoside (AN2) showed highest in vitro antioxidant activity (IC50?DPPH?=?25.27???0.02??g/mL and IC50?ABTS?=?10.71???0.01??g/mL). Anticancer and immunostimulatory activities of cyanidin-3-glucoside (AN1), cyanidin-3-O-?-d-(6-(E)-coumaroyl) glucopyranoside (AN2), delphinidin-3-O-?-d-(6-(E)-coumaroyl) glucopyranoside (AN3), cyanidin-3-O-(2-O-?-xylopyranosyl-6-O-acetyl)-?-glucopyranoside (AN4) and crude anthocyanin extract (AN5) showed high therapeutic perspective. Anthocyanins AN1?4 and crude extract AN5 showed cytotoxicity on C-6 cancer cells and high relative fluorescence units (RFU) at 200??g/mL suggesting promising apoptosis induction activity as well as influential immunostimulatory potential. Observations demonstrate potential of purple anthocyanins enriched tea clone for exploitation as a nutraceutical product. ? 2017, Association of Food Scientists & Technologists (India).
Description
Keywords
Antioxidants, Cell death, Cloning, Cytotoxicity, Flavonoids, Gene expression, Integrated circuits, Plants (botany), Anthocyanidin reductase, Anthocyanidin synthase, Anti-oxidant activities, Anticancer activities, Apoptosis induction, Expression patterns, Immunostimulatory activities, Nutraceutical products, Anthocyanins
Citation
Joshi, R., Rana, A., Kumar, V., Kumar, D., Padwad, Y. S., Yadav, S. K., & Gulati, A. (2017). Anthocyanins enriched purple tea exhibits antioxidant, immunostimulatory and anticancer activities. Journal of Food Science and Technology, 54(7), 1953-1963. doi: 10.1007/s13197-017-2631-7