Leishmania donovani persistence and circulation causing cutaneous leishmaniasis in unusual-foci of Nepal

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dc.contributor.authorRai, Tinmaya
dc.contributor.authorShrestha, Srijan
dc.contributor.authorPrajapati, Sabita
dc.contributor.authorBastola, Anup
dc.contributor.authorParajuli, Niraj
dc.contributor.authorGhimire, Pragya Gautam
dc.contributor.authorBhandari, Parmananda
dc.contributor.authorPandey, Kishor
dc.contributor.authorJain, Manju
dc.contributor.authorMatlashewski, Greg
dc.contributor.authorBras-Goncalves, Rachel
dc.contributor.authorManandhar, Krishna Das
dc.date.accessioned2024-01-16T14:23:27Z
dc.date.accessioned2024-08-13T10:34:19Z
dc.date.available2024-01-16T14:23:27Z
dc.date.available2024-08-13T10:34:19Z
dc.date.issued2023-07-29T00:00:00
dc.description.abstractCutaneous leishmaniasis cases have increased dramatically in recent years in Nepal. The study offers molecular identification of the Leishmania species using 40 patient�s aspiration biopsy samples, targeting markers kinetoplast minicircle DNA (kDNA) and internal transcribed spacer-1 (ITS1). Among molecularly diagnosed 22 cutaneous leishmaniasis cases, L. donovani complex was identified in 13 instances and L. major in 9 cases. The ITS1 PCR was positive in 12 of the positive nested- kDNA PCR cases (12/22), confirming L. donovani complex in seven of the cases and L. major in five of the cases. In addition, the study conclude that concurrent occurrence of atypical cutaneous infections caused by L. donovani parasite in 59.1% of cases and typical cutaneous infections caused by L. major parasite in 40.9% of cases. A Phylogentic analaysis showed that the detected L. donovani species present null genetic distances from seven references of L. donovani, but slight differences between ITS1 sequences and not grouped into a significant monophyletic cluster. � 2023, The Author(s).en_US
dc.identifier.doi10.1038/s41598-023-37458-6
dc.identifier.issn20452322
dc.identifier.urihttps://doi.org/10.1038/s41598-023-37458-6
dc.identifier.urihttp://10.2.3.109/handle/32116/2939
dc.language.isoen_USen_US
dc.publisherNature Researchen_US
dc.subjectDermatitisen_US
dc.subjectDNA, Kinetoplasten_US
dc.subjectHumansen_US
dc.subjectLeishmania donovanien_US
dc.subjectLeishmaniasis, Cutaneousen_US
dc.subjectNepalen_US
dc.subjectkinetoplast DNAen_US
dc.subjectcutaneous leishmaniasisen_US
dc.subjectdermatitisen_US
dc.subjectgeneticsen_US
dc.subjecthumanen_US
dc.subjectLeishmania donovanien_US
dc.subjectNepalen_US
dc.titleLeishmania donovani persistence and circulation causing cutaneous leishmaniasis in unusual-foci of Nepalen_US
dc.title.journalScientific Reportsen_US
dc.typeArticleen_US
dc.type.accesstypeOpen Accessen_US

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