Biochemical Adaptations in Zea mays Roots to Short-Term Pb2+ Exposure: ROS Generation and Metabolism

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dc.contributor.authorKaur, Gurpreet
dc.contributor.authorKaur, Shubhpreet
dc.contributor.authorSingh,Harminder Pal
dc.contributor.authorBatish, Daizy Rani
dc.contributor.authorKohli, R.K.
dc.contributor.authorRishi, Valbha
dc.date.accessioned2019-05-10T06:42:57Z
dc.date.accessioned2024-08-13T09:45:32Z
dc.date.available2019-05-10T06:42:57Z
dc.date.available2024-08-13T09:45:32Z
dc.date.issued2015
dc.description.abstractThe present study investigated the effect of lead (0, 16, 40 and 80 mg L?1 Pb2+) exposure for 3, 12 and 24 h on root biochemistry in hydroponically grown Zea mays (maize). Pb2+ exposure (80 mg L?1) enhanced malondialdehyde content (239 %�427 %), reactive carbonyl groups (425 %�512 %) and H2O2 (129 %�294 %) accumulation during 3�24 h of treatment, thereby indicating cellular peroxidation and oxidative damage. The quantitative estimations were in accordance with in situ detection of ROS generation (using 2?,7?-dichlorodihydrofluorescein diacetate dye) and H2O2 accumulation. Pb2+ treatment significantly reduced ascorbate and glutathione content during 3�24 h of exposure. On the contrary, levels of non-protein thiols were enhanced by 3�11.8 time over control in response to 16�80 mg L?1 Pb2+ treatment, after 24 h. A dose-dependent induction in ascorbate peroxidase and lipoxygenase enzyme activity was observed in Z. mays roots. The activities of ascorbate-recycling enzymes (dehydroascorbate reductase and monodehydroascorbate reductase) were significantly increased in relation to concentration and duration of Pb2+ treatment. The study concludes that Pb2+-exposure induces ROS-mediated oxidative damage during early period of exposure despite the upregulation of enzymes of ascorbate�glutathione cycle.en_US
dc.identifier.citationKaur, Gurpreet., Kaur, Shubhpreet and Singh,Harminder Pal et. al. (2015) Biochemical Adaptations in Zea mays Roots to Short-Term Pb2+ Exposure: ROS Generation and Metabolism. Bulletin of Environmental Contamination and Toxicology. Vol. 95(2), PP.1564en_US
dc.identifier.doi10.1007/s00128-015-1564-y
dc.identifier.issnPrint- 0007-4861
dc.identifier.issnOnline-1432-0800
dc.identifier.urihttp://10.2.3.109/handle/32116/2362
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs00128-015-1564-y
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.subjectCellular peroxidationen_US
dc.subjectROS accumulationen_US
dc.subjectCell deathen_US
dc.subjectDefense mechanismsen_US
dc.titleBiochemical Adaptations in Zea mays Roots to Short-Term Pb2+ Exposure: ROS Generation and Metabolismen_US
dc.title.journalBulletin of Environmental Contamination and Toxicologyen_US
dc.typeArticleen_US
dc.type.accesstypeOpen Accessen_US

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