Identification of Novel lncRNA in Breast Cancer Cell Line

Abstract

Long non-coding RNA(lncRNA) is RNA transcript having size of more 200 nucleotides and low number of exons are present due to which it expressed at lower level in specific tissues. Mainly the lncRNA is involved in gene regulations such as epigenetic regulation, chromatin remodeling and posttranscriptional regulations.The localization of lncrna is mainly in nucleus but there is considerable amount of lncrna present in cytoplasm. Lncrna can also target the mitochondrial genome. Identification of the targets of lncrna on mitochondrial genome helps to find the relationship between lncrna and mitochondrial functions which further helps in diagnosis and in therapy of the mitochondrial related diseases. One such lncRNA is MIR503HG, which act as a tumor suppressor in breast cancer cell line. Expression of lncRNA MIR503HG was analyzed in normoxic and hypoxic conditions upon the treatments of three different types of drugs on breast cancer cell line. The study of expression level of lncRNA MIR503HG in breast cancer cell line suggest a new cancer biomarker.