Identification and cross-species amplification of microsatellite markers derived from expressed sequence data of rose species

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dc.contributor.authorSharma, Ram Kumar
dc.contributor.authorChaudhary, Anil
dc.contributor.authorSharma, Himanshu
dc.contributor.authorBhardwaj, Pankaj
dc.contributor.authorSharma, Vikas
dc.contributor.authorKumar, Rahul
dc.contributor.authorAhuja, Paramveer Singh
dc.date.accessioned2018-01-05T07:44:07Z
dc.date.accessioned2024-08-13T11:04:09Z
dc.date.available2018-01-05T07:44:07Z
dc.date.available2024-08-13T11:04:09Z
dc.date.issued2015
dc.description.abstractGenic SSR markers derived from public expressed sequence tags (ESTs) data are valuable and cost effective marker resources for genome mapping and diversity studies. Owing to their derivation from the transcribed regions which often have putative functions, these markers can be easily associated with desired trait. In the present study, 19 novel SSR markers were identified from 450 non redundant unigenes derived from 3,726 public ESTs of two rose species. Among SSRs, tri-repeats (61.3?%) were most abundant followed by di-repeat (29?%). Newly identified EST-SSR markers recorded significant homology with the known/putative proteins of Arabidopsis thaliana. The cross transferability to 12 rose species ranged from 63.2 to 100?%. Novel SSR loci found to be moderately to highly polymorphic with locus wise average number of alleles and polymorphism information content (PIC) were 4.1 and 0.33, respectively. Cloning and sequencing of EST-SSR size variant amplicons of marker locus Rches12 revealed that the variation in the number of SSR repeat-units was the main source of fragment polymorphism. The high polymorphic potential coupled with high cross-transferability rate demonstrates wider applicability of novel SSR markers in genetic diversity, genome mapping and evolutionary studies in various rose species. ? 2014, Society for Plant Biochemistry and Biotechnology.en_US
dc.identifier.citationSharma, R. K., Chaudhary, A., Sharma, H., Bhardwaj, P., Sharma, V., Kumar, R., & Ahuja, P. S. (2015). Identification and cross-species amplification of microsatellite markers derived from expressed sequence data of rose species. Journal of Plant Biochemistry and Biotechnology, 24(3), 359-364. doi: 10.1007/s13562-014-0287-1en_US
dc.identifier.doi10.1007/s13562-014-0287-1
dc.identifier.issn9717811
dc.identifier.urihttp://10.2.3.109/handle/32116/493
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs13562-014-0287-1
dc.language.isoenen_US
dc.publisherSpringer Indiaen_US
dc.titleIdentification and cross-species amplification of microsatellite markers derived from expressed sequence data of rose speciesen_US
dc.title.journalJournal of Plant Biochemistry and Biotechnology
dc.typeArticleen_US

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