Pseudomonas citronellolis alleviates arsenic toxicity and maintains cellular homeostasis in chickpea (Cicer arietinum L.)

dc.contributor.authorAdhikary, Arindam
dc.contributor.authorSaini, Rashmi
dc.contributor.authorKumar, Rashpal
dc.contributor.authorSingh, Inderjit
dc.contributor.authorRamakrishna, Wusirika
dc.contributor.authorKumar, Sanjeev
dc.date.accessioned2024-01-16T14:23:13Z
dc.date.accessioned2024-08-13T10:34:10Z
dc.date.available2024-01-16T14:23:13Z
dc.date.available2024-08-13T10:34:10Z
dc.date.issued2022-05-19T00:00:00
dc.description.abstractArsenic is a hazardous metalloid that causes detrimental effects on plant growth and metabolism. Plants accumulate arsenic in edible parts that consequently enter the food chain leading to many health problems. Metal tolerant plant growth-promoting bacteria (PGPB) ameliorate heavy metal toxicity. In this study, the effect of arsenic (As5+) and the role of PGPB Pseudomonas citronellolis (PC) in mitigating As5+ toxicity and associated metabolic alterations in chickpea were assessed. Five chickpea varieties (PBG1, GPF2, PDG3, PDG4 and PBG5) were evaluated for arsenic accumulation, translocation, and its interference with metabolic and defense processes. As5+ (40 mg kg?1) interfered with plant metabolism and enhanced the antioxidative and carbohydrate metabolizing enzyme's activity but PC treatment maintained the activity at par with control. PC also facilitated the accumulation of As5+ in the root system and restricted its translocation to the shoot. Further, to map the metabolic changes, Gas chromatography Mass Spectroscopy (GC-MS) based metabolite profiling and gene expression analysis (qRT-PCR) were performed in the best and worst-performing chickpea varieties (PBG1 and PBG5). 48 metabolites of various metabolic pathways (amino acid, carbohydrate, and fatty acid) were altered in As5+ and PC treatment. Gene expressions showed correlation with biochemical analysis of the antioxidative enzymes and carbohydrate metabolizing enzymes while PC treatment improved chlorophyll biosynthesis enzyme CaDALA expression in As5+ treated plants. Therefore, PC mitigates As5+ toxicity by restricting it in the roots thereby maintaining the cellular homeostasis under As5+ stress in chickpeas. � 2022 Elsevier Masson SASen_US
dc.identifier.doi10.1016/j.plaphy.2022.05.014
dc.identifier.issn9819428
dc.identifier.urihttps://doi.org/10.1016/j.plaphy.2022.05.014
dc.identifier.urihttps://kr.cup.edu.in/handle/32116/2877
dc.language.isoen_USen_US
dc.publisherElsevier Masson s.r.l.en_US
dc.subjectAntioxidative enzymesen_US
dc.subjectArsenicen_US
dc.subjectCarbohydrate metabolismen_US
dc.subjectChickpeaen_US
dc.subjectPseudomonas citronellolis (PC)en_US
dc.titlePseudomonas citronellolis alleviates arsenic toxicity and maintains cellular homeostasis in chickpea (Cicer arietinum L.)en_US
dc.title.journalPlant Physiology and Biochemistryen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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