Preparation, development and characterization of Leucaena leucocephala galactomannan (LLG) conjugated sinapic acid: A potential colon targeted prodrug

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dc.contributor.authorKaur, Jasleen
dc.contributor.authorMehta, Vikrant
dc.contributor.authorKaur, Gurpreet
dc.date.accessioned2024-01-21T10:53:59Z
dc.date.accessioned2024-08-14T07:40:45Z
dc.date.available2024-01-21T10:53:59Z
dc.date.available2024-08-14T07:40:45Z
dc.date.issued2021-02-22T00:00:00
dc.description.abstractSinapic acid (SA), a widely prevalent hydroxycinnamic acid, possess numerous biological activities owing to its antioxidant property. The present study was aimed to prepare colon targeted polysaccharidic/polymeric ester prodrug of SA (a microbially triggered system) using Leucaena leucocephala galactomannan (LLG) as a polysaccharidic carrier. The polymeric conjugates of SA-LLG were found to exhibit an increase in % yield and DS with increase in amount of SA and volume of thionyl chloride. The degree of depolymerization of SA-LLG prodrug batches were evaluated using optimized concentration of galactomannase. The SA-LLG prodrug was characterized employing UV and FTIR spectroscopy, 1H NMR and XRD. In vitro release study of the optimized prodrug batch (SL10) suggested stable nature of SA-LLG conjugate under acidic (pH 1.2) and alkaline conditions (pH 6.8). The treatment of prodrug with galactomannase (15 mg/mL) followed by esterase (10 U/mL) enzyme released approximately 81% of SA after 24 h. The cell viability results revealed that free SA and SA-LLG were found to have similar antiproliferative potential against human colon cancer cell lines (HCT-116 cells). Our investigation revealed that polysaccharidic prodrug, SA-LLG, has the potential for colon targeting of SA and thus can be employed for the treatment of Inflammatory Bowel Diseases (IBDs). � 2021 Elsevier B.V.en_US
dc.identifier.doi10.1016/j.ijbiomac.2021.02.132
dc.identifier.issn1418130
dc.identifier.urihttp://10.2.3.109/handle/32116/4174
dc.identifier.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0141813021004232
dc.language.isoen_USen_US
dc.publisherElsevier B.V.en_US
dc.subjectEnzymatic degradationen_US
dc.subjectEsteraseen_US
dc.subjectGalactomannaseen_US
dc.subjectLeucaena leucocephala galactomannanen_US
dc.subjectPolymeric prodrugen_US
dc.subjectSinapic aciden_US
dc.titlePreparation, development and characterization of Leucaena leucocephala galactomannan (LLG) conjugated sinapic acid: A potential colon targeted prodrugen_US
dc.title.journalInternational Journal of Biological Macromoleculesen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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Human Genetics And Molecular Medicine - Research Publications