A study of mechanistic mapping of novel SNPs to male breast cancer

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Date

2019

Authors

Kaur, R.P
Kumar, V
Shafi, G
Vashistha, R
Kulharia, Mahesh
Munshi, Aanjan

Journal Title

Journal ISSN

Volume Title

Publisher

Humana Press Inc.

Abstract

Alterations in BRCA2, PALB2, CHEK2, and p53 genes have been identified for their association with male breast cancer in various studies. The incidence of male breast cancer in India is consistent with its global rate. The present study was carried out with an aim to evaluate the genetic alterations in male breast cancer patients from Malwa region of Punjab, India. Four male breast cancer patients belonging to different families were recruited from Guru Gobind Singh Medical College and Hospital, Faridkot, India. A total of 51 genes reported with implications in the pathogenesis of breast cancer were screened using next generation sequencing. Germline variations were found in BRCA1, BRCA2, PMS2, p53, and PALB2 genes, previously reported to be associated with MBC as well as FBC. In addition to these, 13 novel missense alterations were detected in eight genes including STK11, FZR1, PALB2, BRCA2, NF2, BAP1, BARD1, and CHEK2. Impact of these missense alterations on structure and function of protein was also analyzed through molecular dynamics simulation. Structural analysis of these single nucleotide polymorphisms (SNPs) revealed significant impact on the encoded protein functioning. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

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Keywords

Germline variations, Male breast cancer, Molecular dynamics simulation, Novel missense alterations, SNPs

Citation

Kaur, R.P., Kumar, V.,and Shafi, G.et.al.A study of mechanistic mapping of novel SNPs to male breast cancer.36(8).10.1007/s12032-019-1290-0