An in vitro study ascertaining the role of H2O2 and glucose oxidase in modulation of antioxidant potential and cancer cell survival mechanisms in glioblastoma U-87 MG cells

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dc.contributor.authorCholia, Ravi P.
dc.contributor.authorKumari, Sanju
dc.contributor.authorKumar, Saurabh
dc.contributor.authorKaur, Manpreet
dc.contributor.authorKaur, Manbir
dc.contributor.authorKumar, Raj
dc.contributor.authorDhiman, Monisha
dc.contributor.authorMantha, Anil K.
dc.date.accessioned2013-01-11T14:13:35Z
dc.date.accessioned2024-08-13T13:21:42Z
dc.date.available2013-01-11T14:13:35Z
dc.date.available2024-08-13T13:21:42Z
dc.date.issued2017
dc.description.abstractGlial cells protect themselves from the elevated reactive oxygen species (ROS) via developing unusual mechanisms to maintain the genomic stability, and reprogramming of the cellular antioxidant system to cope with the adverse effects. In the present study non-cytotoxic dose of oxidants, H2O2 (100??M) and GO (10??U/ml) was used to induce moderate oxidative stress via generating ROS in human glioblastoma cell line U-87 MG cells, which showed a marked increase in the antioxidant capacity as studied by measuring the modulation in expression levels and activities of superoxide dismutase (SOD1 and SOD2) and catalase (CAT) enzymes, and the GSH content. However, pretreatment (3?h) of Curcumin and Quercetin (10??M) followed by the treatment of oxidants enhanced the cell survival, and the levels/activities of the antioxidants studied. Oxidative stress also resulted in an increase in the nitrite levels in the culture supernatants, and further analysis by immunocytochemistry showed an increase in iNOS expression. In addition, phytochemical pretreatment decreased the nitrite level in the culture supernatants of oxidatively stressed U-87 MG cells. Elevated ROS also increased the expression of COX-2 and APE1 enzymes and pretreatment of Curcumin and Quercetin decreased COX-2 expression and increased APE1 expression in the oxidatively stressed U-87 MG cells. The immunocytochemistry also indicates for APE1 enhanced stress-dependent subcellular localization to the nuclear compartment, which advocates for enhanced DNA repair and redox functions of APE1 towards survival of U-87 MG cells. It can be concluded that intracellular oxidants activate the key enzymes involved in antioxidant mechanisms, NO-dependent survival mechanisms, and also in the DNA repair pathways for glial cell survival in oxidative-stress micro-environment. ? 2017, Springer Science+Business Media, LLC.en_US
dc.identifier.citationCholia, R. P., Kumari, S., Kumar, S., Kaur, M., Kaur, M., Kumar, R., . . . Mantha, A. K. (2017). An in vitro study ascertaining the role of H<inf>2</inf>O<inf>2</inf>and glucose oxidase in modulation of antioxidant potential and cancer cell survival mechanisms in glioblastoma U-87 MG cells. Metabolic Brain Disease, 32(5), 1705-1716. doi: 10.1007/s11011-017-0057-6en_US
dc.identifier.doi10.1007/s11011-017-0057-6
dc.identifier.issn8857490
dc.identifier.urihttp://10.2.3.109/handle/32116/369
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs11011-017-0057-6
dc.language.isoenen_US
dc.publisherSpringer New York LLCen_US
dc.subjectApe1 Proteinen_US
dc.subjectCatalaseen_US
dc.subjectCopper Zinc Superoxide Dismutaseen_US
dc.subjectCurcuminen_US
dc.subjectCyclooxygenase 2en_US
dc.subjectGlucose Oxidaseen_US
dc.subjectGlutathioneen_US
dc.subjectHydrogen Peroxideen_US
dc.subjectInducible Nitric Oxide Synthaseen_US
dc.subjectManganese Superoxide Dismutaseen_US
dc.subjectNitriteen_US
dc.subjectQuercetinen_US
dc.subjectUnclassified Drugen_US
dc.subjectAntioxidant Activity Cancer Cell Cultureen_US
dc.subjectCell Survivalen_US
dc.subjectCellular Distributionen_US
dc.subjectControlled Studyen_US
dc.subjectDrug Cytotoxicityen_US
dc.subjectEnzyme Linked Immunosorbent Assayen_US
dc.subjectGlioblastomaen_US
dc.subjectHumanen_US
dc.subjectHuman Cellen_US
dc.subjectImmunocytochemistryen_US
dc.subjectIn Vitro Studyen_US
dc.subjectProtein Expressionen_US
dc.subjectWestern Ben_US
dc.titleAn in vitro study ascertaining the role of H2O2 and glucose oxidase in modulation of antioxidant potential and cancer cell survival mechanisms in glioblastoma U-87 MG cellsen_US
dc.title.journalMetabolic Brain Disease
dc.typeArticleen_US

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