MSMEG-5850, a stress-induced TetR protein, involved in global transcription regulation in Mycobacterium smegmatis

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dc.contributor.authorSingh, Parul
dc.contributor.authorKumar, Arbind
dc.contributor.authorChhabra, Ravindresh
dc.contributor.authorSingh, Kashmir
dc.contributor.authorKaur, Jagdeep
dc.date.accessioned2024-01-16T14:23:26Z
dc.date.accessioned2024-08-13T10:34:18Z
dc.date.available2024-01-16T14:23:26Z
dc.date.available2024-08-13T10:34:18Z
dc.date.issued2023-06-07T00:00:00
dc.description.abstractAim: To decipher the role of MSMEG-5850 in the physiology of mycobacteria. Methods: MSMEG-5850 was knocked out and RNA sequencing was performed. MSMEG-5850 protein was purified from the Escherichia coli pET28a system. Electrophoretic mobility shift assay and size exclusion chromatography were used to determine the binding of MSMEG-5850 to its motif and binding stoichiometry. The effect of nutritional stress was monitored. Results: Transcriptome analysis revealed the differential expression of 148 genes in an MSMEG-5850 knockout strain. MSMEG-5850 had control over 50 genes because those genes had a binding motif upstream of their sequence. The electrophoretic mobility shift assay showed MSMEG-5850 bound to its motif as a monomer. MSMEG-5850 was upregulated under nutritional stress and promoted the survival of mycobacteria. Conclusion: The study confirms the role of MSMEG-5850 in global transcriptional regulation. � 2023 Future Medicine Ltd.en_US
dc.identifier.doi10.2217/fmb-2022-0238
dc.identifier.issn17460913
dc.identifier.urihttps://doi.org/10.2217/fmb-2022-0238
dc.identifier.urihttp://10.2.3.109/handle/32116/2932
dc.language.isoen_USen_US
dc.publisherNewlands Press Ltden_US
dc.subjectEMSAen_US
dc.subjectgene expressionen_US
dc.subjectMSMEG-5850en_US
dc.subjectMycobacteriumen_US
dc.subjectTetR-like transcription factorsen_US
dc.subjecttranscriptome analysisen_US
dc.titleMSMEG-5850, a stress-induced TetR protein, involved in global transcription regulation in Mycobacterium smegmatisen_US
dc.title.journalFuture Microbiologyen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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