Isolation And Determination Of Antibiotic Susceptibility Of Enterococcus Faecalis From Malwa Region

Abstract

About 20 isolates of Enterococcus faecalis is isolated from six different places of Malwa region. They include isolate from Animal waste soil, Agriculture soil, Sewage soil and public place soil also. Isolation of Enterococcus faecalis from environment causes concern for diseases i.e. Endocarditis, UTI, neonatal infections due to maintaince of unhygienic conditions. The biochemical tests are performed and measure as presumptive results for Enterococcus faecalis. Antibiotic susceptibility test is performed to observe the susceptibility of strains for different antibiotics. Six different types of antibiotics such as Ampicillin, Piperacillin, Tetracycline, Rifampin, Streptomycin, and Gentamycin are used for Antibiotic Susceptibility test. Antibiotic Susceptibility test analyse that Gentamycin and Streptomycin are found more resistant towards Enterococcus faecalis than others in Malwa region. The susceptibility of strains for antibiotics are different due to the IS elements which are horizontal transfer to the other Enterococcus faecalis also formed biofilm which makes it more antibiotic resistant due to slower growth rate, metabolic activities and poor penetration rate.