Substitution of carbonate by non-physiological synergistic anion modulates the stability and iron release kinetics of serum transferrin

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dc.contributor.authorKumar, Rajesh
dc.contributor.authorSharma, Deepak
dc.contributor.authorKumar, Navinder
dc.contributor.authorKumari, Beeta
dc.contributor.authorShabnam
dc.contributor.authorKumar, Sanjeev
dc.date.accessioned2024-01-21T10:33:04Z
dc.date.accessioned2024-08-13T11:16:17Z
dc.date.available2024-01-21T10:33:04Z
dc.date.available2024-08-13T11:16:17Z
dc.date.issued2022-10-15T00:00:00
dc.description.abstractSerum transferrin (sTf) is a bi-lobal protein. Each lobe of sTf binds one Fe3+ ion in the presence of a synergistic anion. Physiologically, carbonate is the main synergistic anion but other anions such as oxalate, malonate, glycolate, maleate, glycine, etc. can substitute for carbonate in vitro. The present work provides the possible pathways by which the substitution of carbonate with oxalate affects the structural, kinetic, thermodynamic, and functional properties of blood plasma sTf. Analysis of equilibrium experiments measuring iron release and structural unfolding of carbonate and oxalate bound diferric-sTf (Fe2sTf) as a function of pH, urea concentration, and temperature reveal that the structural and iron-centers stability of Fe2sTf increase by substitution of carbonate with oxalate. Analysis of isothermal titration calorimetry (ITC) scans showed that the affinity of Fe3+ with apo-sTf is enhanced by substituting carbonate with oxalate. Analysis of kinetic and thermodynamic parameters measured for the iron release from the carbonate and oxalate bound monoferric-N-lobe of sTf (FeNsTf) and Fe2sTf at pH 7.4 and pH 5.6 reveals that the substitution of carbonate with oxalate inhibits/retards the iron release via increasing the enthalpic barriers. � 2022 Elsevier B.V.en_US
dc.identifier.doi10.1016/j.bbapap.2022.140856
dc.identifier.issn15709639
dc.identifier.urihttp://10.2.3.109/handle/32116/3255
dc.identifier.urlhttps://linkinghub.elsevier.com/retrieve/pii/S1570963922001030
dc.language.isoen_USen_US
dc.publisherElsevier B.V.en_US
dc.subjectActivation enthalpyen_US
dc.subjectIron releaseen_US
dc.subjectReduction-induced iron releaseen_US
dc.subjectStability of iron centersen_US
dc.subjectStructural stabilityen_US
dc.subjectSynergistic anionsen_US
dc.titleSubstitution of carbonate by non-physiological synergistic anion modulates the stability and iron release kinetics of serum transferrinen_US
dc.title.journalBiochimica et Biophysica Acta - Proteins and Proteomicsen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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