Effect of imidazolium based ionic liquids on CO-association dynamics and thermodynamic stability of Ferrocytochrome c

dc.contributor.authorGarg, Mansi
dc.contributor.authorKumar, Sandeep
dc.contributor.authorKaur, Anupamjeet
dc.contributor.authorGoyal, Bhupesh
dc.contributor.authorKumar, Rajesh
dc.date.accessioned2024-01-21T10:32:54Z
dc.date.accessioned2024-08-13T11:16:36Z
dc.date.available2024-01-21T10:32:54Z
dc.date.available2024-08-13T11:16:36Z
dc.date.issued2020-11-05T00:00:00
dc.description.abstractAnalysis of kinetic and thermodynamic parameters measured for CO-association reaction of Ferrocytochrome c (Ferrocyt c) under variable concentrations of 1-butyl-3-methylimidazolium with varying anion ([Bmim]X) (X = Cl?, I?, Br?, HSO4?) at pH 7 revealed that the low concentration of [Bmim]X (?0.5 M) constrains the CO-association dynamics of Ferrocyt c and typically follows the order: [Bmim]HSO4 > [Bmim]Cl > [Bmim]Br > [Bmim]I. At relatively higher concentrations (>0.5), the chaotropic action of [Bmim]+ dominates which consequently increases the thermal-fluctuations responsible to denature the protein and thus accelerates the speed of CO-association reaction. Analysis of thermal denaturation curves of Ferrocyt c measured at different concentrations of [Bmim]X revealed that the [Bmim]X decreases the thermodynamic stability of protein and typically follows the order: [Bmim]I > [Bmim]Br > [Bmim]Cl > [Bmim]CH3COO > [Bmim]HSO4, demonstrating that the effect of [Bmim]X on thermodynamic stability of protein is not in accordance to Hofmeister series effect of anions because instead of increasing the kosmotropic anion carrying [Bmim]X ([Bmim]CH3COO and [Bmim]HSO4) also decreases the thermodynamic stability of protein. � 2020 Elsevier B.V.en_US
dc.identifier.doi10.1016/j.bpc.2020.106497
dc.identifier.issn3014622
dc.identifier.urihttps://kr.cup.edu.in/handle/32116/3204
dc.identifier.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0301462220302052
dc.language.isoen_USen_US
dc.publisherElsevier B.V.en_US
dc.subject1-butyl-3-methylimidazoliumen_US
dc.subjectCO-association dynamicsen_US
dc.subjectFerrocytochrome cen_US
dc.subjectIonic liquiden_US
dc.subjectThermodynamic stabilityen_US
dc.titleEffect of imidazolium based ionic liquids on CO-association dynamics and thermodynamic stability of Ferrocytochrome cen_US
dc.title.journalBiophysical Chemistryen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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