Effect of imidazolium based ionic liquids on CO-association dynamics and thermodynamic stability of Ferrocytochrome c
dc.contributor.author | Garg, Mansi | |
dc.contributor.author | Kumar, Sandeep | |
dc.contributor.author | Kaur, Anupamjeet | |
dc.contributor.author | Goyal, Bhupesh | |
dc.contributor.author | Kumar, Rajesh | |
dc.date.accessioned | 2024-01-21T10:32:54Z | |
dc.date.accessioned | 2024-08-13T11:16:36Z | |
dc.date.available | 2024-01-21T10:32:54Z | |
dc.date.available | 2024-08-13T11:16:36Z | |
dc.date.issued | 2020-11-05T00:00:00 | |
dc.description.abstract | Analysis of kinetic and thermodynamic parameters measured for CO-association reaction of Ferrocytochrome c (Ferrocyt c) under variable concentrations of 1-butyl-3-methylimidazolium with varying anion ([Bmim]X) (X = Cl?, I?, Br?, HSO4?) at pH 7 revealed that the low concentration of [Bmim]X (?0.5 M) constrains the CO-association dynamics of Ferrocyt c and typically follows the order: [Bmim]HSO4 > [Bmim]Cl > [Bmim]Br > [Bmim]I. At relatively higher concentrations (>0.5), the chaotropic action of [Bmim]+ dominates which consequently increases the thermal-fluctuations responsible to denature the protein and thus accelerates the speed of CO-association reaction. Analysis of thermal denaturation curves of Ferrocyt c measured at different concentrations of [Bmim]X revealed that the [Bmim]X decreases the thermodynamic stability of protein and typically follows the order: [Bmim]I > [Bmim]Br > [Bmim]Cl > [Bmim]CH3COO > [Bmim]HSO4, demonstrating that the effect of [Bmim]X on thermodynamic stability of protein is not in accordance to Hofmeister series effect of anions because instead of increasing the kosmotropic anion carrying [Bmim]X ([Bmim]CH3COO and [Bmim]HSO4) also decreases the thermodynamic stability of protein. � 2020 Elsevier B.V. | en_US |
dc.identifier.doi | 10.1016/j.bpc.2020.106497 | |
dc.identifier.issn | 3014622 | |
dc.identifier.uri | https://kr.cup.edu.in/handle/32116/3204 | |
dc.identifier.url | https://linkinghub.elsevier.com/retrieve/pii/S0301462220302052 | |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier B.V. | en_US |
dc.subject | 1-butyl-3-methylimidazolium | en_US |
dc.subject | CO-association dynamics | en_US |
dc.subject | Ferrocytochrome c | en_US |
dc.subject | Ionic liquid | en_US |
dc.subject | Thermodynamic stability | en_US |
dc.title | Effect of imidazolium based ionic liquids on CO-association dynamics and thermodynamic stability of Ferrocytochrome c | en_US |
dc.title.journal | Biophysical Chemistry | en_US |
dc.type | Article | en_US |
dc.type.accesstype | Closed Access | en_US |