Mitochondria-Targeted Small Peptide, SS31 Ameliorates Diabetes Induced Mitochondrial Dynamics in Male TallyHO/JngJ Mice

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dc.contributor.authorBhatti, Jasvinder Singh
dc.contributor.authorThamarai, Kavya
dc.contributor.authorKandimalla, Ramesh
dc.contributor.authorManczak, Maria
dc.contributor.authorYin, Xiangling
dc.contributor.authorKumar, Subodh
dc.contributor.authorVijayan, Murali
dc.contributor.authorReddy, P. Hemachandra
dc.date.accessioned2024-01-21T10:53:59Z
dc.date.accessioned2024-08-14T07:40:45Z
dc.date.available2024-01-21T10:53:59Z
dc.date.available2024-08-14T07:40:45Z
dc.date.issued2020-10-07T00:00:00
dc.description.abstractThe escalating burden of type 2 diabetes (T2D) and its related complications has become a major public health challenge worldwide. Substantial evidence indicates that T2D is one of the culprits for the high prevalence of Alzheimer�s disease (AD) in diabetic subjects. This study aimed to investigate the possible mitochondrial alterations in the pancreas induced by hyperglycemia in diabetes. We used a diabetic TallyHO/JngJ (TH) and non-diabetic, SWR/J mice strains. The diabetic and non-diabetic status in animals was assessed by performing intraperitoneal glucose tolerance test at four time points, i.e., 4, 8, 16, and 24�weeks of age. We divided 24-week-old TH and SWR/J mice into 3 groups: controls, diabetic TH mice, and diabetic TH mice treated with SS31 peptide. After the treatment of male TH mice with SS31, intraperitoneally, for 4�weeks, we studied mitochondrial dynamics, biogenesis, and function. The mRNA and protein expression levels of mitochondrial proteins were evaluated using qPCR and immunoblot analysis. The diabetic mice after 24�weeks of age showed overt pancreatic injury as demonstrated by disintegration and atrophy of ? cells with vacuolization and reduced islet size. Mitochondrial dysfunction was observed in TH mice, as evidenced by significantly elevated H2O2 production, lipid peroxidation, and reduced ATP production. Furthermore, mRNA expression and immunoblot analysis of mitochondrial dynamics genes were significantly affected in diabetic mice, compared with controls. However, treatment of animals with SS31 reduced mitochondrial dysfunction and restored most of the mitochondrial functions and mitochondrial dynamics processes to near normal in TH mice. In conclusion, mitochondrial dysfunction is established as one of the molecular events that occur in the pathophysiology of T2D. Further, SS31 treatment may confer protection against the mitochondrial alterations induced by hyperglycemia in diabetic TallyHO/JngJ mice. � 2020, Springer Science+Business Media, LLC, part of Springer Nature.en_US
dc.identifier.doi10.1007/s12035-020-02142-7
dc.identifier.issn8937648
dc.identifier.urihttp://10.2.3.109/handle/32116/4172
dc.identifier.urlhttps://link.springer.com/10.1007/s12035-020-02142-7
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.subjectAlzheimer�s diseaseen_US
dc.subjectMitochondrial dysfunctionen_US
dc.subjectOxidative stressen_US
dc.subjectTallyHO/JngJ miceen_US
dc.subjectType 2 diabetesen_US
dc.titleMitochondria-Targeted Small Peptide, SS31 Ameliorates Diabetes Induced Mitochondrial Dynamics in Male TallyHO/JngJ Miceen_US
dc.title.journalMolecular Neurobiologyen_US
dc.typeArticleen_US
dc.type.accesstypeOpen Accessen_US

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