Pseudomonas aeruginosa quorum sensing molecule N-3-oxo-dodecanoyl-L-homoserine lactone activates human platelets through intracellular calcium-mediated ROS generation

Simple item page
dc.contributor.authorYadav,V.K.
dc.contributor.authorSingh, P.K..
dc.contributor.authorKalia, M.
dc.contributor.authorSharma, D.
dc.contributor.authorSingh, S.K.
dc.contributor.authorAgarwal, V.
dc.date.accessioned2018-08-24T03:50:22Z
dc.date.accessioned2024-08-13T13:22:07Z
dc.date.available2018-08-24T03:50:22Z
dc.date.available2024-08-13T13:22:07Z
dc.date.issued2018
dc.description.abstractPseudomonas aeruginosa, an opportunistic pathogen release N-3-oxo-dodecanoyl-L-homoserine lactone (3-oxo-C12HSL) and N-butyryl-L-homoserine lactone (C4-HSL) quorum sensing (QS) molecules to regulate various virulence factors responsible for infection in the host. 3-oxo-C12 HSL not only regulates the bacterial gene expression but also modulates the host cell system. Thus, it is pertinent to evaluate the effect of these QS molecules on blood platelets which is responsible for the maintenance of hemostasis and thrombus formation. Here, in the present study, we showed that 3-oxo-C12 HSL activates platelets in a dose-dependent manner and induces intracellular calcium-mediated reactive oxygen species (ROS) release, whereas no such effect was observed with C4-HSL. 3-oxo-C12 HSL stimulated ROS release was mediated by NADPH oxidase. Results confirmed the involvement of phospholipase C (PLC) and IP3 receptor behind intracellular calcium-mediated ROS generation. The impact of 3-oxo-C12 HSL on platelet activation suggests that it could interfere and alter the normal function of platelet in individuals infected with P. aeruginosa.en_US
dc.identifier.citationYadav, Vivek Kumar., Singh, Pradeep Kumar and Kalia, Manmohit et. Al. (2018) Pseudomonas aeruginosa quorum sensing molecule N-3-oxo-dodecanoyl-l-homoserine lactone activates human platelets through intracellular calcium-mediated ROS generation. International Journal of Medical Microbiology. 10.1016/j.ijmm.2018.07.009en_US
dc.identifier.doi10.1016/j.ijmm.2018.07.009
dc.identifier.issn14384221
dc.identifier.urihttp://10.2.3.109/handle/32116/1567
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1438422118300365?via%3Dihub
dc.language.isoenen_US
dc.publisherElsevier GmbHen_US
dc.subjectIntracellular calcium; Platelets; Quorum sensing; ROSen_US
dc.titlePseudomonas aeruginosa quorum sensing molecule N-3-oxo-dodecanoyl-L-homoserine lactone activates human platelets through intracellular calcium-mediated ROS generationen_US
dc.title.journalInternational Journal of Medical Microbiology
dc.typeArticle in Pressen_US

Files

Collections

Zoology - Research Publications