Studies on adaptive environmental responses in Himalayan Rhododendron arboreum

Simple item page
dc.contributor.authorChoudhary, Shruti
dc.contributor.supervisorBhardwaj, Pankaj
dc.date.accessioned2020-08-21T06:36:35Z
dc.date.accessioned2024-08-13T11:02:43Z
dc.date.available2020-08-21T06:36:35Z
dc.date.available2024-08-13T11:02:43Z
dc.date.issued2019
dc.description.abstractTemperate plants acclimatize to survive freezing temperatures, which are otherwise prerequisite in the initiation/transition of a developmental phase. The dominance of Rhododendron arboreum under a highly fluctuating Himalayan environment makes it enticing for genetic structure and functional analysis. In the present study, transcript, small RNA and metabolome libraries from flowers and foliar tissues of reproductive and vegetative seasons were analyzed. The high-quality paired-end reads were assembled into 157,427 non-redundant transcripts and categorized functionally based on gene ontology, pathway, and transcription factor database. The screening for molecular markers identified 35,419 SSR and 811 high-quality SNPs. A comparison of transcript profiles for the vegetative and flowering season tissues revealed that 12,577 unigenes with fluctuating expression were responsible for seasonal adaptations. Additional to the gene interaction networks, 421 ions obtained from LC-MS were annotated to distinct pathways, especially secondary metabolites. Thirdly, 466 conserved and novel miRNAs, 442 precursors, and 27,139 targets were predicted and the miRNAs modulating circadian clock and reproductive development were discussed further. Other than the genes, miRNAs, and compounds held for an active metabolism, signaling, development, and their regulations, supplementary responses to abiotic/biotic stimuli were induced. A multifaceted response not only sponsored the climatic encounters but brought the shift from vegetative to reproductive growth. The genome-wide profiling and the spatiotemporal variation in mRNA and miRNA expression, as well as the nontargeted metabolome, will enhance the understanding of development and tolerance strategies in high altitude tree species.en_US
dc.identifier.accessionnoT00871
dc.identifier.citationChoudhary, Shruti & Bhardwaj, Pankaj (2019) Studies on adaptive environmental responses in Himalayan Rhododendron arboreumen_US
dc.identifier.urihttp://10.2.3.109/handle/32116/2747
dc.language.isoen_USen_US
dc.publisherCentral University of Punjaben_US
dc.subjectFlowering regulationen_US
dc.subjectCircadian clocken_US
dc.subjectAdaptationen_US
dc.subjectDe novo assemblyen_US
dc.subjectAnnotationen_US
dc.subjectMolecular markersen_US
dc.subjectDifferential expressionen_US
dc.subjectMetabolomeen_US
dc.subjectMicroR profilingen_US
dc.titleStudies on adaptive environmental responses in Himalayan Rhododendron arboreumen_US
dc.typeThesisen_US

Files

Collections

Botany - Research Publications