A candidate triple-negative breast cancer vaccine design by targeting clinically relevant cell surface markers: an integrated immuno and bio-informatics approach

Simple item page
dc.contributor.authorKumar, Shashank
dc.contributor.authorShuaib, Mohd
dc.contributor.authorPrajapati, Kumari Sunita
dc.contributor.authorSingh, Atul Kumar
dc.contributor.authorChoudhary, Princy
dc.contributor.authorSingh, Sangeeta
dc.contributor.authorGupta, Sanjay
dc.date.accessioned2024-01-16T14:23:12Z
dc.date.accessioned2024-08-13T10:34:09Z
dc.date.available2024-01-16T14:23:12Z
dc.date.available2024-08-13T10:34:09Z
dc.date.issued2022-02-20T00:00:00
dc.description.abstractTriple-negative breast cancer (TNBC) is an aggressive, metastatic/invasive sub-class of breast cancer (BCa). Cell surface protein-derived multi-epitope vaccine-mediated targeting of TNBC cells could be a better strategy against the disease. Literature-based identified potential cell surface markers for TNBC cells were subjected to expression pattern and survival analysis in BCa patient sample using TCGA database. The cytotoxic and helper T-lymphocytes antigenic epitopes in the test proteins were identified, selected and fused together with the appropriate linkers and an adjuvant, to construct the multi-epitope vaccine (MEV). The immune profile, physiochemical property (PP) and world population coverage of the MEV was studied. Immune simulation, cloning in a suitable vector, molecular docking (against Toll-like receptors, MHC (I/II) molecules), and molecular dynamics simulations of the MEV was performed. Cell surface markers were differentially expressed in TNBC samples and showed poor survival in TNBC patients. Satisfactory PP and WPC (up to 89 and 99%) was observed. MEV significant stable binding with the immune molecules and induced the immune cells in silico. The designed vaccine has capability to elicit immune response which could be utilized to target TNBC alone/combination with other therapy. The experimental studies are required to check the efficacy of the vaccine. � 2022, King Abdulaziz City for Science and Technology.en_US
dc.identifier.doi10.1007/s13205-022-03140-3
dc.identifier.issn2190572X
dc.identifier.urihttps://doi.org/10.1007/s13205-022-03140-3
dc.identifier.urihttp://10.2.3.109/handle/32116/2869
dc.language.isoen_USen_US
dc.publisherSpringer Science and Business Media Deutschland GmbHen_US
dc.subjectImmune simulationen_US
dc.subjectMolecular dockingen_US
dc.subjectMolecular dynamics simulationen_US
dc.subjectMulti-epitope vaccineen_US
dc.subjectTriple-negative breast canceren_US
dc.titleA candidate triple-negative breast cancer vaccine design by targeting clinically relevant cell surface markers: an integrated immuno and bio-informatics approachen_US
dc.title.journal3 Biotechen_US
dc.typeArticleen_US
dc.type.accesstypeOpen Accessen_US

Files

Collections

Biochemistry And Microbial Sciences - Research Publications