Autoinducer N-(3-oxododecanoyl)-L-homoserine lactone induces calcium and reactive oxygen species-mediated mitochondrial damage and apoptosis in blood platelets

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dc.contributor.authorYadav, Vivek Kumar
dc.contributor.authorSingh, Pradeep Kumar
dc.contributor.authorSharma, Deepmala
dc.contributor.authorPandey, Himanshu
dc.contributor.authorSingh, Sunil Kumar
dc.contributor.authorAgarwal, Vishnu
dc.date.accessioned2024-01-21T10:44:35Z
dc.date.accessioned2024-08-13T13:22:20Z
dc.date.available2024-01-21T10:44:35Z
dc.date.available2024-08-13T13:22:20Z
dc.date.issued2021-02-23T00:00:00
dc.description.abstractAcylated homoserine lactones (AHL) such as N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12 HSL) and N-butyryl-L-homoserine lactone (C4 HSL) are the most common autoinducer molecules in Pseudomonas aeruginosa. These AHL molecules not only regulate the expression of virulence factors but also have been shown to interfere with the host cell and modulate its functions. Recently, we reported that 3-oxo-C12 HSL but not C4 HSL causes cytosolic Ca2+ rise and ROS production in platelets. In this study, we examined the potential of AHLs to induce apoptosis in the human blood platelet. Our result showed that 3-oxo-C12 HSL but not C4 HSL causes phosphatidylserine (PS) exposure, mitochondrial dysfunction (mitochondrial transmembrane potential loss, and mitochondrial permeability transition pore (mPTP) formation). Besides, 3-oxo-C12 HSL also inhibited thrombin-induced platelet aggregation and clot retraction. The pretreatment of an intracellular calcium chelator BAPTA-AM or ROS inhibitor (DPI) significantly attenuated the 3-oxo-C12 HSL induced apoptotic characters such as PS exposure and mitochondrial dysfunctions. These data, including our previous findings, confirmed that 3-oxo-C12 HSL induced intracellular Ca2+ mediated ROS production results in the activation and subsequent induction of apoptotic features in platelets. Our results demonstrated that the 3-oxo-C12 HSL modulates the functions of platelets that may cause severe thrombotic complications in P. aeruginosa infected individuals. � 2021 Elsevier Ltden_US
dc.identifier.doi10.1016/j.micpath.2021.104792
dc.identifier.issn8824010
dc.identifier.urihttp://10.2.3.109/handle/32116/3796
dc.identifier.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0882401021000644
dc.language.isoen_USen_US
dc.publisherAcademic Pressen_US
dc.subjectAcyl-homoserine lactonesen_US
dc.subjectApoptosisen_US
dc.subjectPlateleten_US
dc.subjectPseudomonas aeruginosaen_US
dc.titleAutoinducer N-(3-oxododecanoyl)-L-homoserine lactone induces calcium and reactive oxygen species-mediated mitochondrial damage and apoptosis in blood plateletsen_US
dc.title.journalMicrobial Pathogenesisen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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