Optimization, composition, and antioxidant activities of exo-and intracellular polysaccharides in submerged culture of cordyceps gracilis (Grev.) durieu & Mont.

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dc.contributor.authorSharma, S.K.
dc.contributor.authorGautam, N.
dc.contributor.authorAtri, N.S.
dc.date.accessioned2018-07-14T01:19:00Z
dc.date.accessioned2024-08-14T06:40:20Z
dc.date.available2018-07-14T01:19:00Z
dc.date.available2024-08-14T06:40:20Z
dc.date.issued2015
dc.description.abstractUnder present experiments, EPS and IPS production, monosaccharide composition, and antioxidant activities of C. gracilis were studied for the first time under submerged culture conditions. Effect of different factors on polysaccharides production was studied by orthogonal experiments using one-factor-at-a-time method. Incubation of culture in the medium with capacity 200 mL (675.12 ? 5.01 and 385.20 ? 5.01 mg/L), rotation speed 150 rpm (324.62 ? 3.32 and 254.62 ? 4.62 mg/L), 6-day culture incubation time (445.24 ? 1.11, 216.60 ? 1.71 mg/L), pH 6.0 (374.81 ? 2.52 and 219.45 ? 2.59 mg/L), and temperature 23?C (405.24 ? 1.11 and 215.60 ? 1.71 mg/L) produced higher EPS and IPS, respectively. Maximum EPS and IPS production was observed in the medium supplemented with glucose as a carbon source (464.82 ? 2.12 and 264.42 ? 2.62 mg/L) and yeast extract as a nitrogen source (465.21 ? 3.11 and 245.17 ? 3.24 mg/L), respectively. Carbon to nitrogen ratio for maximum EPS and IPS production was observed as 10: 1 (395.29 ? 2.15 and 235.62 ? 1.40 mg/L), respectively. Glucose was found to be the major monosaccharide (62.15 ? 7.33%). Both EPS and IPS of C. gracilis showed significant DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power, and iron chelating activity. ? 2015 Sapan Kumar Sharma et al.en_US
dc.identifier.citationSharma, S. K., Gautam, N., & Atri, N. S. (2015). Optimization, composition, and antioxidant activities of exo-and intracellular polysaccharides in submerged culture of cordyceps gracilis (Grev.) durieu & Mont. Evidence-based Complementary and Alternative Medicine, 2015. doi: 10.1155/2015/462864en_US
dc.identifier.doi10.1155/2015/462864
dc.identifier.issn1741427X
dc.identifier.urihttp://10.2.3.109/handle/32116/1416
dc.identifier.urlhttps://www.hindawi.com/journals/ecam/2015/462864/
dc.language.isoen_USen_US
dc.publisherHindawi Limiteden_US
dc.subjectAntioxidanten_US
dc.subjectExopolysaccharideen_US
dc.subjectFungal polysaccharideen_US
dc.subjectGlucoseen_US
dc.subjectIntracellular polysaccharideen_US
dc.subjectNitrogenen_US
dc.subjectUnclassified drugen_US
dc.subjectABTS radical scavenging assayen_US
dc.subjectAntioxidant activity carbon nitrogen ratioen_US
dc.subjectCarbon sourceen_US
dc.subjectCordycepsen_US
dc.subjectCordyceps gracilisen_US
dc.subjectDPPH radical scavenging assayen_US
dc.subjectEC50en_US
dc.subjectIncubation timeen_US
dc.subjectNon humanen_US
dc.subjectPHen_US
dc.subjectRotationen_US
dc.subjectYeasten_US
dc.titleOptimization, composition, and antioxidant activities of exo-and intracellular polysaccharides in submerged culture of cordyceps gracilis (Grev.) durieu & Mont.en_US
dc.title.journalEvidence-based Complementary and Alternative Medicineen_US
dc.typeRetracteden_US
dc.type.accesstypeOpen Accessen_US

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