Proteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soil

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dc.contributor.authorDhawi, Faten
dc.contributor.authorDatta, Rupali
dc.contributor.authorRamakrishna, Wusirika
dc.contributor.authorDhawi, F.
dc.contributor.authorDatta, R.
dc.contributor.authorRamakrishna, W.
dc.date.accessioned2018-02-02T10:16:49Z
dc.date.accessioned2024-08-13T10:34:06Z
dc.date.available2018-02-02T10:16:49Z
dc.date.available2024-08-13T10:34:06Z
dc.date.issued2017
dc.description.abstractSorghum is an economically important crop, a model system for gene discovery and a biofuel source. Sorghum seedlings were subjected to three microbial treatments, plant growth promoting bacteria (B), arbuscular mycorrhizal (AM) fungi mix with two Glomus species (G. aggregatum and G. etunicatum), Funelliformis mosseae and Rhizophagus irregularis (My), and B and My combined (My + B). Proteomic analysis was conducted followed by integration with metabolite, plant biomass and nutrient data. Out of 366 differentially expressed proteins in sorghum roots, 44 upregulated proteins overlapping among three treatment groups showed positive correlation with sorghum biomass or element uptake or both. Proteins upregulated only in B group include asparagine synthetase which showed negative correlation with biomass and uptake of elements. Phosphoribosyl amino imidazole succinocarboxamide protein with more than 50-fold change in My and My + B groups correlated positively with Ca, Cu, S and sucrose levels in roots. The B group showed the highest number of upregulated proteins among the three groups with negative correlation with sorghum biomass and element uptake. KEGG pathway analysis identified carbon fixation as the unique pathway associated with common upregulated proteins while biosynthesis of amino acids and fatty acid degradation were associated with common downregulated proteins. Protein-protein interaction analysis using STRING identified a major network with thirteen downregulated proteins. These findings suggest that plant-growth-promoting-bacteria alone or in combination with mycorrhiza enhanced radical scavenging system and increased levels of specific proteins thereby shifting the metabolism towards synthesis of carbohydrates resulting in sorghum biomass increase and uptake of nutrients. ? 2016en_US
dc.identifier.citationDhawi, F., Datta, R., & Ramakrishna, W. (2017). Proteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soil. Biochimica et Biophysica Acta - Proteins and Proteomics, 1865(2), 1-9. doi: 10.1016/j.bbapap.2016.11.015en_US
dc.identifier.doi10.1016/j.bbapap.2016.11.015
dc.identifier.issn15709639
dc.identifier.urihttp://10.2.3.109/handle/32116/570
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1570963916302527?via%3Dihub
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.subjectProteomicsen_US
dc.subjectMetabolitesen_US
dc.subjectPlant growth promoting bacteriaen_US
dc.subjectMycorrhizaen_US
dc.subjectPathwaysen_US
dc.titleProteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soilen_US
dc.title.journalBiochimica et Biophysica Acta - Proteins and Proteomics
dc.typeArticleen_US

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