Structural, kinetic and thermodynamic characterizations of SDS-induced molten globule state of a highly negatively charged cytochrome c
dc.contributor.author | Jain, R | |
dc.contributor.author | Sharma, D | |
dc.contributor.author | Kumar, Rakesh | |
dc.contributor.author | Kumar, Rajesh | |
dc.date.accessioned | 2019-09-03T09:41:19Z | |
dc.date.accessioned | 2024-08-13T11:16:32Z | |
dc.date.available | 2019-09-03T09:41:19Z | |
dc.date.available | 2024-08-13T11:16:32Z | |
dc.date.issued | 2019 | |
dc.description.abstract | This study presents the structural, kinetic and thermodynamic characterizations of previously unknown submicellar concentrations of SDS-induced molten globule (MGSDS) state of a highly negatively charged basedenatured ferricytochrome c (U B -state) at pH ∼12.8 (±0.2). The far-UV CD, near-UV CD, ANS-fluorescence data of UB-state in the presence of different concentrations of SDS indicate that the submicellar concentrations of SDS (≤0.4mM) transform the UBstate to MG SDS -state. The MG SDS -state has nativelike α-helical secondary structure but lacks tertiary structure. The free energy change (ΔG° D) for U B → MG SDS transition determined by far-UV CD (∼2.7 kcal mol -1 ) is slightly higher than those determined by fluorescence (∼2.0 kcal mol -1 ) at 25°C. At very low SDS and NaCl concentrations, the MG SDS -state undergoes cold denaturation. As SDS concentration is increased, the thermal denaturation temperature increases and the cold denaturation temperature decrease. Kinetic experiments involving the measurement of the CO-association rate to the base-denatured ferrocytochrome c at pH ≈12.8 (±0.2), 25°C indicate that the submicellar concentrations of SDS restrict the internal dynamics of base-denatured protein. © The Author(s) 2018. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved. | en_US |
dc.identifier.citation | Jain, R., Sharma, D. and Kumar, R., et.al.Structural, kinetic and thermodynamic characterizations of SDS-induced molten globule state of a highly negatively charged cytochrome c.165(2).PP.125-137.10.1093/jb/mvy087 | en_US |
dc.identifier.doi | 10.1093/jb/mvy087 | |
dc.identifier.issn | 0021924X | |
dc.identifier.uri | https://kr.cup.edu.in/handle/32116/2447 | |
dc.identifier.url | https://academic.oup.com/jb/article/165/2/125/5146343 | |
dc.language.iso | en | en_US |
dc.publisher | Oxford University Press | en_US |
dc.subject | Alkali molten-globule | en_US |
dc.subject | Constrained dynamics | en_US |
dc.subject | Entropic stabilization | en_US |
dc.subject | SDS | en_US |
dc.subject | Thermal stability | en_US |
dc.title | Structural, kinetic and thermodynamic characterizations of SDS-induced molten globule state of a highly negatively charged cytochrome c | en_US |
dc.title.journal | Journal of Biochemistry | en_US |
dc.type | Article | en_US |
dc.type.accesstype | Closed Access | en_US |
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