Structural, kinetic and thermodynamic characterizations of SDS-induced molten globule state of a highly negatively charged cytochrome c

dc.contributor.authorJain, R
dc.contributor.authorSharma, D
dc.contributor.authorKumar, Rakesh
dc.contributor.authorKumar, Rajesh
dc.date.accessioned2019-09-03T09:41:19Z
dc.date.accessioned2024-08-13T11:16:32Z
dc.date.available2019-09-03T09:41:19Z
dc.date.available2024-08-13T11:16:32Z
dc.date.issued2019
dc.description.abstractThis study presents the structural, kinetic and thermodynamic characterizations of previously unknown submicellar concentrations of SDS-induced molten globule (MGSDS) state of a highly negatively charged basedenatured ferricytochrome c (U B -state) at pH ∼12.8 (±0.2). The far-UV CD, near-UV CD, ANS-fluorescence data of UB-state in the presence of different concentrations of SDS indicate that the submicellar concentrations of SDS (≤0.4mM) transform the UBstate to MG SDS -state. The MG SDS -state has nativelike α-helical secondary structure but lacks tertiary structure. The free energy change (ΔG° D) for U B → MG SDS transition determined by far-UV CD (∼2.7 kcal mol -1 ) is slightly higher than those determined by fluorescence (∼2.0 kcal mol -1 ) at 25°C. At very low SDS and NaCl concentrations, the MG SDS -state undergoes cold denaturation. As SDS concentration is increased, the thermal denaturation temperature increases and the cold denaturation temperature decrease. Kinetic experiments involving the measurement of the CO-association rate to the base-denatured ferrocytochrome c at pH ≈12.8 (±0.2), 25°C indicate that the submicellar concentrations of SDS restrict the internal dynamics of base-denatured protein. © The Author(s) 2018. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.en_US
dc.identifier.citationJain, R., Sharma, D. and Kumar, R., et.al.Structural, kinetic and thermodynamic characterizations of SDS-induced molten globule state of a highly negatively charged cytochrome c.165(2).PP.125-137.10.1093/jb/mvy087en_US
dc.identifier.doi10.1093/jb/mvy087
dc.identifier.issn0021924X
dc.identifier.urihttps://kr.cup.edu.in/handle/32116/2447
dc.identifier.urlhttps://academic.oup.com/jb/article/165/2/125/5146343
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.subjectAlkali molten-globuleen_US
dc.subjectConstrained dynamicsen_US
dc.subjectEntropic stabilizationen_US
dc.subjectSDSen_US
dc.subjectThermal stabilityen_US
dc.titleStructural, kinetic and thermodynamic characterizations of SDS-induced molten globule state of a highly negatively charged cytochrome cen_US
dc.title.journalJournal of Biochemistryen_US
dc.typeArticleen_US
dc.type.accesstypeClosed Accessen_US

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