Expression of KIBRA in Breast Cancer Cell Lines

Abstract

Breast cancer the most frequently diagnosed cancer and its metastasis to distant organs accounts the majority of deaths. Numerous genes and proteins are involved in the cause of metastasis. Though KIBRA is one of the component of Hippo Pathway and is reported as tumor suppressor but this scaffolding protein has also been found to be an emerging and important player in the process of metastasis. It has been reported that KIBRA protein interacts with various proteins through its domain and leads cytoskeleton arrangement, cell polarity and migration. N terminal and C terminal of the protein contains the WW, Internal C2 & putative class III PDZ domain that interacts with DDR1, DLC1 & PKC? and helps the breast cancer cells to metastasize. To study whether KIBRA is involved in breast cancer metastasis, we checked its expression at both protein and mRNA level by Immunoblotting and Real Time PCR which showed increased KIBRA expression in ER positive cells. Further investigation to elucidate the role of KIBRA in ER positive cells, ER transfection and immunoblotting in triple negative breast cancer cell lines were performed, which indicated that ER leads to enhanced KIBRA expression in breast cancer cells.