Cloning, characterization and transmission blocking potential of midgut carboxypeptidase A in Anopheles stephensi

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dc.contributor.authorVenkatRao, V.
dc.contributor.authorKumar, S.K.
dc.contributor.authorSridevi, P.
dc.contributor.authorMuley, V.Y.
dc.contributor.authorChaitanya, R.K.
dc.date.accessioned2018-03-13T09:57:55Z
dc.date.accessioned2024-08-13T13:22:03Z
dc.date.available2018-03-13T09:57:55Z
dc.date.available2024-08-13T13:22:03Z
dc.date.issued2017
dc.date.issued2017
dc.description.abstractTransmission-blocking vaccines (TBV) interrupt malaria parasite transmission and hence form an important component for malaria eradication. Mosquito midgut exopeptidases such as aminopeptidase N & carboxypeptidase B have demonstrated TBV potential. In the present study, we cloned and characterized carboxypeptidase A (CPA) from the midgut of an important malarial vector, Anopheles stephensi. ClustalW amino acid alignment and in silico 3-dimensional structure analysis of CPA predicted the presence of active sites involved in zinc and substrate binding that are conserved among all the known mosquito species. Real-time PCR analysis demonstrated that CPA is predominantly expressed in the midgut throughout the mosquito life cycle and that this gene is significantly elevated in P. berghei-infected mosquitoes compared to uninfected blood-fed controls. The high midgut CPA activity correlated with the prominent mRNA levels observed. Peptide-based anti-CPA antibodies were raised that cross-reacted specifically to ?48?kDa and ?37?kDa bands, which correspond to zymogen and active forms of CPA. Further, the addition of CPA-directed antibodies to P. berghei-containing blood meal significantly reduced the mosquito infection rate in the test group compared to control and blocked the parasite development in the midgut. These results support further development of A. stephensi CPA as a candidate TBV. ? 2016 Elsevier B.V.en_US
dc.identifier.citationVenkatRao, V., Kumar, S. K., Sridevi, P., Muley, V. Y., & Chaitanya, R. K. (2017). Cloning, characterization and transmission blocking potential of midgut carboxypeptidase A in Anopheles stephensi. Acta Tropica, 168, 21-28. doi: 10.1016/j.actatropica.2016.12.035en_US
dc.identifier.doi10.1016/j.actatropica.2016.12.035en_US
dc.identifier.doi10.1016/j.actatropica.2016.12.035
dc.identifier.issn0001-706X
dc.identifier.issn0001706X
dc.identifier.urihttp://10.2.3.109/handle/32116/654
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0001706X16304715?via%3Dihub
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.subjectCarboxypeptidase Aen_US
dc.subjectPlasmodium Bergheien_US
dc.subjectMidguten_US
dc.subjectTransmission blocking vaccine (TBV)en_US
dc.titleCloning, characterization and transmission blocking potential of midgut carboxypeptidase A in Anopheles stephensien_US
dc.title.journalActa Tropica
dc.title.journalActa Tropicaen_US
dc.typeArticleen_US

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