Singh, Varun PratapChawda, NityaBarkhade, TejalMahapatra, Santosh KumarBanerjee, Indrani2024-01-212024-08-132024-01-212024-08-132021-04-08885451310.1002/bab.2163http://10.2.3.109/handle/32116/3649Ex vivo interaction of NaYF4:Yb,Er nanophosphors with isolated mitochondria has been investigated. The nanophosphors were synthesized using the hydrothermal method. The synthesized NaYF4:Yb,Er nanophosphors were characterized for physicochemical properties. The NaYF4:Yb,Er nanophosphors showed successful upconversion with excitation wavelength lying in the near-infrared region. The effect of synthesized NaYF4:Yb,Er nanophosphors on mitochondria isolated from the chicken heart tissue was examined through ROS generation capacity, membrane fluidity, and complex II activity. The exposer of NaYF4:Yb,Er nanophosphors to isolated mitochondria inhibits ROS generation activity as compared to control. The mitochondria membrane fluidity of the lipid bilayer and complex-II activity of mitochondria was observed to be unaltered after the interaction with NaYF4:Yb,Er nanoparticles. The results confirm that synthesized NaYF4:Yb,Er nanoparticles can be used as a safe contrast agent. � 2021 International Union of Biochemistry and Molecular Biology, Inc.en-USfluiditymitochondriaorganellesreactive oxygen species (ROS) generationupconversion nanoparticlesArticlehttps://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2163