Kaur, GurpreetSingh, Harminder PalDaizy R. BatishMahajan, PriyankaKohli, R.K.Valbha, Rishi2017-07-242024-08-132017-07-242024-08-132015Kaur, G., Singh, H. P., Batish, D. R., Mahajan, P., Kohli, R. K., & Rishi, V. (2015). Exogenous Nitric Oxide (NO) interferes with lead (Pb)-induced toxicity by detoxifying reactive oxygen species in hydroponically grown wheat (Triticum aestivum) roots. PLoS ONE, 10(9). doi: 10.1371/journal.pone.01387131932620310.1371/journal.pone.0138713https://kr.cup.edu.in/handle/32116/194Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 ?M) alone and in combination with SNP (100 ?M) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure. ? 2015 Kaur et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en-USAlkadieneAntioxidantHydroxyl groupLeadMalonaldehydeNtric oxideNitroprusside sodiumReactive oxygen metaboliteSuperoxideHydrogen peroxideLeadNitric oxideNitroprusside sodiumReactive oxygen metabolite detoxificationEnzyme activityHistochemistryHydroponicsIn vivo studyLead poisoningLong term exposureMembrane damageNon humanOxidative stressPhytotoxicityPlant growthPlant rootTriticum aestivumWheatCell membrane permeabilityDrug effectsArticlehttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138713