Grover, P.Kaur, J.Suri, K.A.Kumar, R.Bansal, G.2024-01-212024-08-132024-01-212024-08-132022-05-039313010.1007/s10600-022-03639-4https://kr.cup.edu.in/handle/32116/3543A new compound, 1,1,2-tris(2?,4?-di-tert-butylphenyl)-4,4-dimethylpent-1-ene, was isolated and characterized from the leaves of Nyctanthes arbor-tristis L. The plant was subjected to bioactivity-guided fractionation, and a compound was isolated from chloroform extract that was found to have potent anticancer activity. The chloroform extract was further fractionated, and a pure compound was isolated that was found to be active against three cancer cell lines (HL-60, HCT-116, and A-549). An RP-HPLC method was developed for quantification of the isolated compound. The content of the isolated compound was 0.88% in the chloroform extract and 0.08% in N. arbor-tristis leaves. � 2022, Springer Science+Business Media, LLC, part of Springer Nature.en-USanticancerextractionfractionationHPLCisolationNyctanthes arbor-tristisspectroscopyArticlehttps://link.springer.com/10.1007/s10600-022-03639-4