Singh, ParulKumar, ArbindChhabra, RavindreshSingh, KashmirKaur, Jagdeep2024-01-162024-08-132024-01-162024-08-132023-06-071746091310.2217/fmb-2022-0238https://doi.org/10.2217/fmb-2022-0238http://10.2.3.109/handle/32116/2932Aim: To decipher the role of MSMEG-5850 in the physiology of mycobacteria. Methods: MSMEG-5850 was knocked out and RNA sequencing was performed. MSMEG-5850 protein was purified from the Escherichia coli pET28a system. Electrophoretic mobility shift assay and size exclusion chromatography were used to determine the binding of MSMEG-5850 to its motif and binding stoichiometry. The effect of nutritional stress was monitored. Results: Transcriptome analysis revealed the differential expression of 148 genes in an MSMEG-5850 knockout strain. MSMEG-5850 had control over 50 genes because those genes had a binding motif upstream of their sequence. The electrophoretic mobility shift assay showed MSMEG-5850 bound to its motif as a monomer. MSMEG-5850 was upregulated under nutritional stress and promoted the survival of mycobacteria. Conclusion: The study confirms the role of MSMEG-5850 in global transcriptional regulation. � 2023 Future Medicine Ltd.en-USEMSAgene expressionMSMEG-5850MycobacteriumTetR-like transcription factorstranscriptome analysisArticle