Tiwari, AbhaySingh, GarimaSingh, UmeshSapra, LeenaRana, VikrantSharma, VasudhaSrivastava, Rupesh K.Sharma, Satyawati2024-01-212024-08-132024-01-212024-08-132021-10-20950542310.1111/ijfs.15410http://10.2.3.109/handle/32116/3078The present review attempts to critically examine and evaluate research findings on mushrooms as sources of vitamin D and other nutraceuticals. Recently, there is a growing concern about diseases associated with the deficiency of vitamin D in humans. As people tend to stay indoors, in present times, due to the COVID-19 pandemic, vitamin D levels are further affected. Research indicates vitamin D as a promising defensive or therapeutic agent against COVID, making this review more crucial. Mushrooms, as a rich source of vitamin D along with various bioactive compounds, perform a significant role in resolving health issues. Robust analyses of various strategies for enhancing vitamin D content in mushrooms holds significance in this study; moreover, this will help stakeholders of the mushroom industry in enriching the overall mushroom quality and human health. Mushroom-based medicinal formulations and functional foods serve to deliver vitamins and nutrients to humans, thus helping to combat malnutrition and other health problems, especially in developing countries. Evidence from pre-clinical and clinical analyses suggests that vitamin D2 bioavailability in mushrooms is comparable with vitamin D from other sources. The review also emphasises molecular findings from mushrooms related to genes responsible for morphology and metabolic production of pro-vitamin-D2. � 2021 Institute of Food Science and Technologyen-USbioavailabilityfortificationmushroomsprovitamin-D₂(ergosterol)sunlightUV-Irradiationvegansvitamin D₂(ergocalciferol)Reviewhttps://ifst.onlinelibrary.wiley.com/doi/10.1111/ijfs.15410