Garg, RohiniSingh, Vikash K.Rajkumar, Mohan SinghKumar, VinayJain, Mukesh2018-03-062024-08-132018-03-062024-08-1320171. Garg R, Singh V, Mohan R, Kumar V, Jain M (2017) Global transcriptome and co-expression network analyses reveal cultivar-specific molecular signatures associated with seed development and seed size/weight determination in chickpea. The Plant Journal doi: 10.1111/tpj.13621.Online - 1365-313X10.1111/tpj.13621https://kr.cup.edu.in/handle/32116/624Seed development is an intricate process regulated via a complex transcriptional regulatory network. To understand the molecular mechanisms governing seed development and seed size/weight in chickpea, we performed a comprehensive analysis of transcriptome dynamics during seed development in two cultivars with contrasting seed size/weight (small-seeded, Himchana 1 and large-seeded, JGK 3). Our analysis identified stage-specific expression for a significant proportion (>13%) of the genes in each cultivar. About one half of the total genes exhibited significant differential expression in JGK 3 as compared with Himchana 1. We found that different seed development stages can be delineated by modules of coexpressed genes. A comparative analysis revealed differential developmental stage specificity of some modules between the two cultivars. Furthermore, we constructed transcriptional regulatory networks and identified key components determining seed size/weight. The results suggested that extended period of cell division during embryogenesis and higher level of endoreduplication along with more accumulation of storage compounds during maturation determine large seed size/weight. Further, we identified quantitative trait loci-associated candidate genes harboring single nucleotide polymorphisms in the promoter sequences that differentiate small- and large-seeded chickpea cultivars. The results provide a valuable resource to dissect the role of candidate genes governing seed development and seed size/weight in chickpea.enCicer arietinumGSE79719GSE79720coexpression networkgene expressionseed size/weighttranscriptional modulestranscriptome dynamicsGlobal transcriptome and coexpression network analyses reveal cultivar-specific molecular signatures associated with seed development and seed size/weight determination in chickpea.Articlehttps://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.13621Plant Journal