Banu J, RajeshUsman T M, MohamedS, KavithaKannah R, YukeshK N, YogalakshmiP, SivashanmugamBhatnagar, AmitKumar, Gopalakrishnan2024-01-212024-08-142024-01-212024-08-142021-02-15360319910.1016/j.ijhydene.2021.01.075http://10.2.3.109/handle/32116/3945The potential to operate energy efficient and less expensive production methods are important in biohydrogen production. Biological hydrogen production is often constrained by less productivity. However, to obtain industrial level implementation, greater productivity is essential. Researches on various bioreactors configurations and influencing factors were deeply investigated in this regard. The bioreactors operated in batch mode are appropriate for preliminary optimization whereas industrial level execution needs continuous mode. The main objective of this review is to recap the limitations and constraints associated with bioreactor operation and to list out the enhancement approaches that are currently investigated for improved biohydrogen generation. Recent approaches designed towards biohydrogen production enhancement such as substrate pre-treatments, inhibitors removal, bioaugmentation, immobilization, effluent recycling, buffering capacity maintenance, exploitation of by-products etc., are reviewed thoroughly. � 2021 Hydrogen Energy Publications LLCen-USAcid rich effluentsBiohydrogenBiomass washoutEnergy and economic analysisInhibitorsArticlehttps://linkinghub.elsevier.com/retrieve/pii/S0360319921001415