Halder, ArindomBiswas, RathindranathKushwaha, Prem PrakashHalder, Krishna KamalAhmed, ImtiazSingh, HarjinderKumar, ShashankHaldar, Krishna Kanta2024-01-162024-08-132024-01-162024-08-132022-02-122191163010.1007/s12668-022-00950-whttps://doi.org/10.1007/s12668-022-00950-whttp://10.2.3.109/handle/32116/2868Biosynthesis of nanostructured materials is an arising feature of the interdisciplinary relationship between nanotechnology and biotechnology and acquiring consideration because of developing interest to foster ecologically favorable innovations in material preparation. In the present study, we synthesized an environmentally friendly and green method for the synthesis of gold/silver bimetallic nanostructure using Eichhornia crassipes leaf extract as reducing and capping agent. Au/Ag nanostructures were characterized by UV�Visible spectroscopy, X-ray photoelectron spectroscopy, and power X-ray diffraction. Transmission electron microscopy images also confirmed the formation of Au/Ag nanostructures. Antibacterial activity of Au/Ag nanostructures was studied and it has been found that Ag/Au nanostructure at 100��M concentration significantly inhibited the bacterial growth of Escherichia coli bacteria. Moreover, the Hoechst 33342 staining method was used to study the effect of Ag/Au nanostructure particles on the morphological changes in breast cancer cell (MDA-MB-231) nucleus. Staining of the Ag/Au nanostructure particle�treated MDA-MB-231 cells (4�h treatments) showed the appearance of emblematic features of apoptosis such as cell membrane blabbing and shrinkage. Graphical Abstract: [Figure not available: see fulltext.]. � 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.en-USAntibacterialAu/AgBimetallic nanostructureEichhornia crassipesArticle