Sarkar, Bibekananda2020-08-212024-08-132020-08-212024-08-132018Sarkar, Bibekananda, Mantha, Anil K. and Mittal, Sunil (2018) ORGANOPHOSPHATE PESTICIDES PESTER Aβ- INDUCED GENOTOXIC RESPONSES IN CULTURED NEURONAL CELLS: APE1/Ref-1 MEDIATED INTERVENTIONhttp://10.2.3.109/handle/32116/2718Amyloid beta ( ) peptide deposition is the primary cause of neurodegeneration in reasons deposition, but the actual cause is not apparent. Several reports point towards the role of pesticides in the AD pathogenesis, especially organophosphate pesticides (OPPs) that also act as acetylcholinesterase inhibitors (AChEIs) and are reported to be neurotoxic in nature at sub-lethal doses. Monocrotophos (MCP) and Chlorpyrifos (CP) are the most widely used OPPs with highest production and consumption throughout the world. - induced oxidative stress associated with the neurodegeneration in AD has been assessed -35) peptide. Natural compounds like curcumin have been well documented for their ameliorating powers against various neurodegenerative disease models. The cell survival assay showed that MCP and CP kill the neuronal cells in both dose- and time-dependently. Nitro blue tetrazolium (NBT) based assay for determination of intracellular reactive oxygen species (ROS) demonstrated that MCP and CP produce significant oxidative stress in IMR-32 and SH-SY -35) increased oxidative stress in IMR-32 and SH-SY5Y cells, whereas curcumin reduced ROS levels significantly (pen-USAPE1; Nrf2; Curcumin; D damage.ThesisT00592