Das, AnamikaSingh, JatinderYogalakshmi, K. N.2017-07-252024-08-142017-07-252024-08-142016Anamika Das, Jatinder Randhawa and Yogalakshmi. K.N. (2016), “Comparative analysis of Laccase immobilization on magnetic iron nanoparticles using two activating agents: EDAC and Cyanuric Chloride”, Indian Journal of Natural Sciences, Vol 6, Issue 35 Pp 10608-10615.0976 –0997http://10.2.3.109/handle/32116/203Surface modification improves the covalent bonding of enzymes onto the magnetic nanoparticles. The present study aims to evaluate the effect of surface activators (EDAC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide) and Cyanuric chloride) in immobilization of enzymes. Nanoparticles prepared by co-precipitation method ranged insize from 15-20 nm. The nanoparticles possessed crystalline property as confirmed by the XRD (X-ray powder diffraction) peaks. SEM-EDS (Scanning Electron Microscopy- Energy Dispersive X-ray Spectroscopy) analysis of EDAC and cyanuric acid activated nanoparticles showed atomic sulphur percent of 0.08% and 0.02%, respectively. It is concluded that EDACwas more successful in loading more enzymes than cyanuric acid. Bradford estimation of the unbound protein after first wash for ENP-EDAC and ENP-CC was 29.1 μg/mL and 132.1 μg/mL, respectively. EDAC is a potential surface modifier for enzyme immobilization process.en-USImmobilizationEDACCyanuric Chloride (CC)EnzymeNanoparticlesLaccaseArticle