Kaur, GurpreetSingh, SandeepJain, A.K.2017-08-102024-08-142017-08-102024-08-1420171. Kaur G, Jain AK and Singh S. Pesticide Induced Cytotoxicity Analysis in Human Cells. Journal of Agroecology and Natural Resource Management
. 2017; 4(1):44-48.Print- 2394-0786Online- 2394-0794http://10.2.3.109/handle/32116/325The aim of our work was to evaluate the effect of pesticides on cell survival, oxidative stress mechanism in hPBMC (human Peripheral Blood Mononucleated Cells) and lung cancer cells (A549 cell line). Cells were exposed to each atrazine, butachlor, chlorpyriphos and Dichlorvos pesticides of varying concentration individually and in combination of pesticides to evaluate pesticide- pesticide interaction. The hPBMNc and A549 cancer cells have been exposed to 1, 10, 50, 100 μg/ml concentration of each atrazine, butachlor, chlorpyriphos and Dichlorvos for 24 h exposure period. The cytotoxicity study revealed that the atrazine, butachlor, chlorpyriphos and Dichlorvos resulted in statistically significant decrease in cell survival rate in dose dependent manner in hPBMC and A549 cells. The cell cycle arrest has also been observed following 10 μg/ml concentration in cancer cells. The oxidative stress analysis has been done to evaluate the toxicity mechanism behind the cytotoxicity of pesticides under investigation. Our results suggest the increased oxidative stress, and cell cytotoxicity in both hPBMC and A549 Cells. The combined treatments of various pesticides suggest their combinatorial and/or synergistic impact on human cells.en-USReactive Oxygen SpeciesCell CycleCytotoxicityOxidative StressArticle