Tyagi, M.Chandran, A.Joshi, T.Prakash, J.Agrawal, V.V.Biradar, A.M.2018-01-102024-08-132018-01-102024-08-1320142014Tyagi, M., Chandran, A., Joshi, T., Prakash, J., Agrawal, V. V., & Biradar, A. M. (2014). Self assembled monolayer based liquid crystal biosensor for free cholesterol detection. Applied Physics Letters, 104(15). doi: 10.1063/1.4871704Print- 0003-6951Online- 1077-31183695110.1063/1.4871704https://kr.cup.edu.in/handle/32116/538A unique cholesterol oxidase (ChOx) liquid crystal (LC) biosensor, based on the disruption of orientation in LCs, is developed for cholesterol detection. A self-assembled monolayer (SAM) of Dimethyloctadecyl[3-(trimethoxysilyl)propyl] ammonium chloride (DMOAP) and (3-Aminopropyl)trimethoxy-silane (APTMS) is prepared on a glass plate by adsorption. The enzyme (ChOx) is immobilized on SAM surface for 12 h before utilizing the film for biosensing purpose. LC based biosensing study is conducted on SAM/ChOx/LC (5CB) cells for cholesterol concentrations ranging from 10mg/dl to 250 mg/dl. The sensing mechanism has been verified through polarizing optical microscopy, scanning electron microscopy, and spectrometric techniques. ? 2014 AIP Publishing LLC.enChlorine compoundsCholesterolLiquid crystalsScanning electron microscopySelf assembled monolayersAmmonium chlorideCholesterol detectionCholesterol oxidaseFree cholesterolsLiquid crystal biosensorsPolarizing optical microscopySensing mechanismSpectrometric techniquesBiosensorsArticlehttps://aip.scitation.org/doi/10.1063/1.4871704