Kaur, G.Cholia, R.P.Joshi, G.Amrutkar, S.M.Kalra, S.Mantha, Anil K.Banerjee, U.C.Kumar, Raj2018-07-142024-08-132018-07-142024-08-132018Kaur, G., Cholia, R. P., Joshi, G., Amrutkar, S. M., Kalra, S., Mantha, A. K., . . . Kumar, R. (2018). Anticancer activity of dihydropyrazolo[1,5-c]quinazolines against rat C6 glioma cells via inhibition of topoisomerase II. Archiv Der Pharmazie, 351(6). doi: 10.1002/ardp.201800023365623310.1002/ardp.201800023https://kr.cup.edu.in/handle/32116/1233The design and synthesis of dihydropyrazolo[1,5-c]quinazolines (1a?h) as human topoisomerase II (TopoII) catalytic inhibitors are reported. The compounds were investigated for their antiproliferative activity against the C6 rat glial cell line. Two compounds, 1b and 1h, were found to be potent cytotoxic agents against glioma cells and exerted selective TopoII inhibitory activity. Furthermore, the compounds induced alterations in reactive oxygen species levels as measured by DCFDA assay and were found to induce cell cycle arrest at the G1 phase at lower concentrations and profound apoptosis at higher concentrations. The interaction of selected investigational molecules with TopoII was further corroborated by molecular modeling. ? 2018 Deutsche Pharmazeutische Gesellschaften-US2 methoxy 4 (2 phenyl 5,6 dihydropyrazolo[1,5 c]quinazolin 5 yl)phenol2 methoxy 5 (2 phenyl 5,6 dihydropyrazolo[1,5 c]quinazolin 5 yl)phenol2 phenyl 5,6 dihydropyrazolo[1,5 c]quinazoline 5 carboxylic acid4 (2 phenyl 5,6 dihydropyrazolo[1,5 c]quinazolin 5 yl)benzonitrile4 (2 phenyl 5,6 dihydropyrazolo[1,5 c]quinazolin 5 yl)phenol4 [2 (2 chlorophenyl) 5,6 dihydropyrazolo[1,5 c]quinazolin 5 yl)phenolcytotoxic agentdihydropyrazolo[1,5 c]quinazoline derivativeDNA topoisomerase (ATP hyArticlehttps://onlinelibrary.wiley.com/doi/abs/10.1002/ardp.201800023