| dc.contributor.author | Santra, Sneha | |
| dc.date.accessioned | 2018-08-31T04:13:36Z | |
| dc.date.available | 2018-08-31T04:13:36Z | |
| dc.date.issued | 2018 | |
| dc.identifier.citation | Sanitra, Sneha (2018) Development of lactadherin based electrochemical
biosensor for the detection of platelet microvesicles | en_US |
| dc.identifier.uri | http://kr.cup.edu.in/handle/32116/1791 | |
| dc.description.abstract | Platelet derived microvesicles (PMVs) are the most abundant membrane vesicles in
the blood having a potent pro-inflammatory effect, promote coagulation and affect
vascular function which are involved in the pathogenesis of cardiovascular disease
including diabetes, thrombosis, and coronary artery diseases. Therefore, it is
pertinent to detect PMVs level in blood of an individual which have prognostic
potential for cardiovascular diseases. As per literature available, lactadherin is a small
(53-66 kDa) multifunctional glycoprotein which plays an important role in the
clearance of microvesicles. In the present study, lactadherin based electrochemical
biosensor for the detection of PMVs was explored. Polythionine film (as a good
electron mediator) was electrochemically deposited on ITO-coated glass through
electrochemical process involving cyclic voltammetry (CV) and chronoamperometry.
Electrochemically deposited electrode provides ideal adsorbing platform for
immobilization of RGDS peptide sequences (Lactadherine binding motif) having
binding affinity against active conformation of integrin on PMVs surface. CV and
Diffusion Pulse Voltammetry (DPV) measurements showed gradual decrease of
v
current with the subsequent adsorption of microvesicles poor plasma (MPP),
microvesicles rich plasma (MRP) and activated platelets. Decrease of current clearly
depicts the presence of microvesicles in blood plasma. Our developed fabricated
electrode can have a promising potential for its efficient application in clinical testing
of various pathological conditions. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | Central University of Punjab | en_US |
| dc.subject | Platelets | en_US |
| dc.subject | Microvesicles | en_US |
| dc.subject | Lactadherin | en_US |
| dc.subject | Cardiovascular disease | en_US |
| dc.subject | Electrochemical biosensor | en_US |
| dc.title | Development of lactadherin based electrochemical
biosensor for the detection of platelet microvesicles | en_US |
| dc.type | Master Dissertation | en_US |
| dc.contributor.supervisor | Singh, Sunil | |
| dc.identifier.accessionno | T00608 | |
