Expression analysis of long non-coding RNA GAS5 and BANCR in lung cancer cell line A549 compared to IMR-90.
Loading...
Files
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Central University of Punjab
Abstract
Lung cancer is the major cause of death worldwide. Several chemotherapeutic drugs and therapies have been established, but the early diagnosis and prognosis of lung cancer is still a question. Long non-coding RNAs are important regulator molecules in the human genome, which serves as transcriptional modulator, post transcriptional processor, chromatin remodeletor and splicing regulator during the gene modification process. Emerging studies have suggested the role of long noncoding RNA as potential biomarker for cancer diagnosis and prognosis by functioning as tumor suppressors and oncogenes. Several studies have been reported on cell lines, tissues as well as tumor and the molecular mechanism is still not clearly understood. GAS5 and BANCR are two long non-coding RNAs, which are found to be down-regulated in multiple cancers such as lung carcinoma, breast cancer, etc. In the current study, we focus on the expression analysis of GAS5 and BANCR in A549 cell line compared to IMR-90 cell line to study the role of long non-coding RNA in the pathogenesis of lung cancer. Furthermore, we investigated the expression of GAS5 and BANCR using quantitative Real-Time PCR. The result showed that GAS5 and BANCR expression was significantly down-regulated in cancerous cell line compared to non-cancerous cell line. The fold change of lncRNAs GAS5 and BANCR was 14 times (P=0.0088) and 7 times (P=0.0088) down-regulated in A549 cell line respectively. The melt curve analysis showed that there was only one sharp peak obtained for both GAS5 and BANCR i iv which suggests that primers bind to their specific targets and no primer dimer was observed.
Description
Keywords
Long non-coding RNA, GAS5, BANCR, Quantitative Real- Time PCR, Melt curve
Citation
Sharma, Uttam (2018) - Expression analysis of long non-coding RNA GAS5 and BANCR in lung cancer cell line A549 compared to IMR-90.