• Login
    View Item 
    •   Knowledge Repository Home
    • School of Basic and Applied Sciences
    • Department of Pharmaceutical Sciences and Natural Products
    • Pharmaceutical Sciences and Natural Products-Master Dissertation
    • View Item
    •   // Knowledge Repository Home
    • // School of Basic and Applied Sciences
    • // Department of Pharmaceutical Sciences and Natural Products
    • // Pharmaceutical Sciences and Natural Products-Master Dissertation
    • // View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Knowledge RepositorySchools & CollectionsBy Issue DateAuthorsTitlesSubjectsBy Submit DateThis CollectionBy Issue DateAuthorsTitlesSubjectsBy Submit Date

    My Account

    LoginRegister

    Phytochemical investigation and biological evaluation of secondary metabolites from asparagus racemosus l through in-vitro and in-silico approach

    Thumbnail
    View/Open
    T00070.pdf (4.843Mb)
    Date
    2013
    Author
    Singla, Ramit
    Metadata
    Show full item record
    Abstract
    Nature has been a source of medicinal products for millennia, and with many useful drugs developed from different natural resources, with majority of drugs are from plant origin. Asparagus racemosus belonging to family liliaceae, is one such important medicinal plant. This plant species is used traditionally in India and other parts of the world in epilepsy, Vaat disorders, brain tonic, hypertension, hepatoprotection, immunostimulant and antiabortifacient. In this context, the aim of the present study was to explore the roots of A.racemosus in terms of its medicinal values for instances antimutagenic, and advanced glycation end-product inhibitor. Antimutagenic activity of different extracts were evaluated using Ames test. A. racemosus methanolic extract (RME) and aqueous extract (RAE) have been found to have effective in the inhibition mutation induced by NPD and sodium azide. Among the two extracts, RAE and RME showed maximum inhibition of 49.2%, and 40.63% in Co-incubation mode respectively. The inhibition of BSA-glucose for the determination of antiglycation activity showed that the inhibition varied significantly among different extracts of A. racemosus. The highest inhibition measured by BSA-glucose was observed for (Ethyl acetate extract) REE (IC50 37.56 ± 1.65 ?g/mL) followed by (methanolic extract) RME (IC50 51.32 ± 1.48 ?g/mL). Isolation of molecules from methanol extract led to the characterisation of one molecule v namely nyasol out of total seven isolated molecules. The molecular docking study of isolated molecule Nyasol displayed strong binding affinity with estrogen receptor ? and estrogen receptor ?, indicating that Nyasol is beneficial in hormone responsive breast cancer. Moreover, in-silico study of already reported phytoestrogens from A.racemosus was also carried out using Glide docking to investigate interaction pattern with Human placental estrone sulphatases (1P49), human 17?-hydroxysteroid-dehydrogenase type 1 (1FDS), human glucose 6-phosphate dehydrogenase (2BH9) and tubulin protein receptors. The top docking score was obtained for rutin (estrogen receptor ?), 3,6,4'-trimethoxy-7-O-?-D-glucopyranosyl [1?4]-O-?-D-xylopyranoside glucopyranpsyl (HSP90), 8-Methoxy-5,6,4-trihydroxyisoflavone-7-O-?-D-glucopyranoside (human placental estrone sulphatase), Shatavarin X (17?-hydroxydehydrogenase`), Racemoside A (Glucose-6-phosphate dehydrogenase), Immunoside (Colchicine binding site of tubulin). The results indicated that phytoestrogens are likely potential candidate for controlling tumor progression with a special emphasis in breast cancer progression.
    URI
    http://210.212.34.21/handle/32116/1924
    Collections
    • Master's [191]
    • Pharmaceutical Sciences and Natural Products-Master Dissertation [25]

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Initiatives by University Library 
    Central University of Punjab
     

     


    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Initiatives by University Library 
    Central University of Punjab